Cholecystokinin- and gastrin-induced protein and amylase secretion from the parotid gland of the anaesthetized rat

Regulatory Peptides
Hülya Cevik Aras, J Ekström

Abstract

I.V. infusion of pentagastrin (20 microg/kg/h) or cholecystokinin (CCK)-8 (1 microg/kg/h) for 10 min caused secretion of salivary proteins from the parotid gland in the anaesthetized rat without any accompanying overt fluid secretion. This "occult" response was revealed by a subsequent wash-out injection of methacholine (5 microg/kg, I.V.) 10 min after the end of the infusion period (aiming at avoiding synergistic interactions). While the fluid response to methacholine was unaffected by the preceding infusion of pentagastrin and CCK-8, the output of protein increased by 147% (pentagastrin) and 74% (CCK-8) and that of amylase by 45% (CCK-8) compared to the responses to methacholine upon saline infusion. Those increases were abolished by the CCK-A receptor blocker (lorglumide), but not by the CCK-B receptor blocker (itriglumide). Evisceration, combined sympathetic and parasympathetic denervation of the glands and assay under adrenoceptor blockade excluded contribution from the gastro-intestinal tract, central or ganglionic mechanisms and circulating catecholamines to the increase in protein/amylase. Furthermore, Western blot demonstrated CCK receptors for both A and B subtypes in normal and chronically denervated glands. In the s...Continue Reading

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Citations

May 13, 2014·Journal of Immunological Methods·Amal DafarHülya Cevik-Aras
Jan 24, 2014·Obesity Research & Clinical Practice·Hirotaka UedaAkio Inui
Jul 22, 2008·Journal of Pineal Research·Hülya Cevik Aras, Jörgen Ekström
Jun 8, 2018·Journal of Oral Rehabilitation·A M L PedersenJ Ekström
Mar 15, 2012·Journal of Anatomy·Francesco LoyJörgen Ekström
Feb 13, 2018·Autoimmunity Reviews·Kashi Raj BhattaraiHan-Jung Chae

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