Cholecystokinin activates area postrema neurons in rat brain slices

The American Journal of Physiology
K Sun, A V Ferguson

Abstract

Peripheral cholecystokinin (CCK) reduces food intake and triggers the secretion of both oxytocin and corticotropin-releasing hormone. These responses are partially initiated by activation of receptors in the peripheral endings of the vagus nerve. However, in vivo studies showing that after vagotomy systemic CCK induces fos activation of neurons in the area postrema (AP) suggest that circulating CCK may directly influence the activity of neurons in this structure. The present study was therefore designed to investigate the responsiveness of AP neurons to CCK using in vitro extracellular single-unit recording techniques. Bath application of 100 nM CCK for 200 s resulted in excitatory responses in 41% and inhibitory effects in 6% of 143 AP neurons tested. Application of multiple doses of CCK (1-100 nM) to single neurons demonstrated that CCK effects were dose dependent. The firing rate of tested neurons increased by 48 +/- 15% in response to 1 nM, by 89 +/- 22% in response to 10 nM, and by 242 +/- 77% in response to 100 nM CCK. After we blockaded synaptic transmission with a low-Ca2+/high-Mg2+ artificial cerebrospinal fluid, the excitatory effects of CCK remained in all nine neurons tested. The CCK-receptor antagonist L-364,718 ha...Continue Reading

Citations

Nov 1, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Marion DanzerPeter Holzer
Jun 16, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shuxia WanR Alberto Travagli
May 14, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Pauline M Smith, Alastair V Ferguson
Feb 15, 2011·Experimental Physiology·Charles C T HindmarchDavid Murphy
Dec 14, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Zhongling ZhengR Alberto Travagli
Jan 17, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Al-Shaimaa F AhmedKeith A Sharkey
Jan 13, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gregory M HolmesR Alberto Travagli
Oct 16, 2001·The Journal of Physiology·J A SánchezS S Sheu
Nov 29, 2005·Neuroscience·J A Russell, P J Brunton

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.