Cholecystokinin (CCK) and CCK receptor expression by human gliomas: Evidence for an autocrine/paracrine stimulatory loop

Neuropeptides
Eftychia OikonomouEric F Adams

Abstract

Cholecystokinin (CCK) is a gut-brain peptide has been described to be able to induce mitosis according to recent studies. Additionally, conflicting data has been published on whether tumours of the central and peripheral nervous system in general, and gliomas in particular, express CCK receptors. In the present in vitro study we employed reverse transcription followed by the polymerase chain reaction (RT-PCR) to investigate whether mRNA for CCK-A and CCK-B receptors as well as CCK peptide itself is present in primary human gliomas and the U-87 MG GBM cell line. The data show that 14/14 (100%) of the primary gliomas exhibited mRNA expression for the CCK peptide gene and the B receptor including the U-87 MG cells, whereas, only 2/14 (14%) showed presence of the CCK-A receptor. The presence of CCK receptors together with CCK peptide expression itself suggests presence of an autocrine loop controlling glioma cell growth. In support of this conclusion, a neutralizing antibody against the CCK peptide exhibited a dose dependent inhibition of cell growth whereas, antagonists to CCK caused a dose depend inhibition of exogenous stimulated glioma cell growth in vitro, via the CCK-B receptor which is PKC activated. Assessment of apoptosis ...Continue Reading

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Citations

Jun 15, 2010·Bioorganic & Medicinal Chemistry·Séverine BrillouetSandrine Silvente-Poirot
May 19, 2016·Scandinavian Journal of Gastroenterology·Jens F RehfeldLinda Bardram
Apr 30, 2017·Frontiers in Endocrinology·Jens F Rehfeld
Dec 17, 2014·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Charity Wayua, Philip S Low

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