PMID: 6987659Jan 1, 1980Paper

Cholecystokinin-converting enzymes in brain

Proceedings of the National Academy of Sciences of the United States of America
A MalesciR S Yalow

Abstract

Crude extracts of porcine cerebral cortical tissue convert cholecystokinin (CCK) to its COOH-terminal fragments, the dodecapeptide (CCK-12) and the octapeptide (CCK-8). The Sephadex G-75 void volume eluate of the crude extract cleaves the arginine-isoleucine bond and effects conversion only to CCK-12; the Sephadex G-50 void volume eluate of the same extract cleaves the arginine-aspartate bond as well, so that both CCK-12 and CCK-8 are end products. Thus, there are at least two enzymes; the one involved in the conversion to CCK-12 is of larger molecular radius than the other. The Km for the cleavage of CCK at the arginine-isoleucine bond by the Sephadex G-75 void volume eluate enzyme is 1.1 X 10(-6) M; the Km for trypsin cleavage of the same bond is 4.7 x 10(-6) M. The lower Vmax for the brain enzyme (1.5 x 10(-11) mol/min per g of extract) compared with trypsin (66 x 10(-11) mol/min per g of trypsin) simply reflects the lesser degree of purify of the brain extract than of the highly purified trypsin.

References

Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J E MullerR S Yalow
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E StrausR S Yalow

Citations

Jan 1, 1981·Journal of Neural Transmission·M FeketeG Telegdy
Dec 12, 1983·Brain Research·J A FinkelsteinM Praissman
Jul 15, 1994·Brain Research. Developmental Brain Research·M G Oakes, T P Davis
Jan 1, 1984·Regulatory Peptides·R W Steigerwalt, J A Williams
Mar 1, 1990·Peptides·C M TurkelsonJ Hamilton
Jan 1, 1983·Progress in Neuro-psychopharmacology & Biological Psychiatry·S LalH Guyda
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S W RyderR S Yalow
Dec 1, 1981·Gut·G B Irvine, R F Murphy
Sep 1, 1985·Journal of Neurochemistry·L SteardoT N Chase
Jan 1, 1985·Annals of the New York Academy of Sciences·N R Goltermann
Jul 3, 1989·FEBS Letters·R E Rodríguez, M P Sacristán

Related Concepts

Brain
CCK-33
Peptide Fragments
Peptide Hydrolases
Substrate Specificity

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