PMID: 9188808May 22, 1997Paper

Cholecystokinin regulates glycoprotein membrane composition of rat pancreatic zymogen granules

Biochimica Et Biophysica Acta
I De DiosM A Manso

Abstract

Lectin-binding studies were performed on rat pancreatic zymogen granules to investigate the alterations in the carbohydrate membrane composition under both chronic CCK stimulation and long-term CCK blockade for 3, 7 and 15 days. By flow cytometry using FITC-WGA--which specifically binds to N-acetylglucosamine and sialic acid--we measured the amount of WGA molecules bound to each individual granule. Parallel studies on pancreatic secretion were also carried out. CCK treatment displayed a differential effect on two zymogen granule subpopulations (Z1 and Z2) identified by flow cytometry on the basis of their light scatter properties: no effects on Z2 zymogen granules were observed in CCK-treated rats, while Z1 granules showed a significant increase in WGA binding at day + 7 which coincides with an increase in protein secretion in response to the hormone. On the contrary, a significant decrease in the amount of WGA receptors was observed in zymogen granule membrane of both the Z1 and Z2 subsets of rats subjected to a long-term CCK blockade. Again, these changes parallel to the reduction observed in protein secretion. Our results suggest that glycoconjugates of zymogen granule membrane involved in CCK-regulated exocytosis contain N-...Continue Reading

References

Nov 1, 1992·Experimental Cell Research·C López-MediavillaJ M Medina
Apr 15, 1994·Biochemical and Biophysical Research Communications·A C WagnerJ A Williams
Dec 24, 1997·Molecular and Cellular Endocrinology·A C García-MonteroI de Dios
Dec 19, 2000·Biochimica Et Biophysica Acta·I de DiosM A Manso

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Citations

Dec 24, 1997·Molecular and Cellular Endocrinology·A C García-MonteroI de Dios
Jun 3, 1999·European Journal of Clinical Investigation·A C García-MonteroI De Dios
Dec 19, 2000·Biochimica Et Biophysica Acta·I de DiosM A Manso

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