PMID: 7370750Apr 21, 1980Paper

Cholecystokinins in rat cerebral cortex: identification, purification and characterization by immunochemical methods

Brain Research
G J Dockray

Abstract

Cholecystokinins in rat cerebral cortex were studied by radioimmunoassay using an antiserum specific for the COOH-terminus of CCK8. Total concentrations of immunoreactive CCK in cortex were 3-4 fold higher than in jejunum. Rat cerebral CCK was purified by immuno-affinity adsorption to the IgG fraction of CCK antisera conjugated to Sepharose beads, and by gel filtration and ion exchange chromatography. Over 90% of immunoreactive CCK in cortex was accounted for by a factor with the properties of synthetic CCK8, and by a closely related slightly less acidic peptide. In contrast, intestinal CCK consisted of about equal amounts of CCK8-like activity and a larger less acidic immunoreactive component. In both cortex and intestine CCK8-like activity was obtained in substantially higher yield after extracting at neutral pH in boiling water than after extracting in either 0.2 M HCl or 0.5 M acetic acid. However, the larger molecular weight forms of CCK in the intestine were recovered in similar yield by acid and neutral extraction. The principal large form of rat CCK was distinguishable from porcine CCK33 on the basis of both optimum extraction conditions and chromatographic properties. It is suggested that the different distribution of ...Continue Reading

References

Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R E MainsN Ling
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J E MullerR S Yalow
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E StrausR S Yalow
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R B InnisS H Snyder
Jan 1, 1979·Annual Review of Physiology·G J Dockray
Apr 1, 1979·Journal of Neurochemistry·J F Rehfeld, N R Goltermann
Aug 12, 1977·Science·D A Price, M J Greenberg

❮ Previous
Next ❯

Citations

Apr 22, 1982·Brain Research·M C Beinfeld, M Palkovits
Jun 11, 1982·Journal of Immunological Methods·J B Jansen, C B Lamers
Jun 8, 1984·Journal of Immunological Methods·J B Jansen, C B Lamers
Nov 16, 1984·Journal of Immunological Methods·R F MurphyS N Joffe
Nov 1, 1988·Brain Research. Developmental Brain Research·D PelapratM Peschanski
Jan 1, 1984·Regulatory Peptides·R W Steigerwalt, J A Williams
Jan 1, 1981·Peptides·E StrausR S Yalow
Jul 1, 1983·Peptides·R Dimaline
Jan 1, 1989·Neurochemistry International·K TakedaT Kato
Jun 1, 1993·Neurochemistry International·M RaiteriF Vallebuona
Jan 1, 1986·Progress in Neuro-psychopharmacology & Biological Psychiatry·N WatanabeT Moroji
Oct 1, 1981·Neuroscience Letters·N N OsborneG J Dockray

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.