Cholera and pertussis toxins reveal multiple regulation of cAMP levels in the rabbit carotid body

The European Journal of Neuroscience
T G CacheroC Gonzalez

Abstract

It is known that hypoxia (PO2 approximately equal to 66-18 mm Hg), acting via unknown receptors, increases carotid body cAMP levels in Ca(2+)-free solutions, indicating that low PO2 activates adenylate cyclases independently of the action of the released neurotransmitters. The aim of the present work was to investigate the involvement of G proteins in the genesis of the basal level of cAMP and on the increase in cAMP induced by low PO2. In carotid body homogenates, cholera toxin- and pertussis toxin-induced [32P]ADP-ribosylation of two protein bands of approximately equal to 42 and 45 kDa, and approximately equal to 39 and 40 kDa respectively; in both cases, prior incubation of the carotid bodies with the toxins reduced [32P]ADP-ribosylation by > 90%. In intact carotid bodies, cholera toxin treatment increased cAMP levels more in normoxic than in hypoxic organs, indicating that hypoxia releases neurotransmitters acting on receptors negatively coupled to adenylate cyclases. Cholera toxin-treated carotid bodies incubated in Ca(2+)-free solution had identical cAMP levels in normoxia and in hypoxia. In pertussis toxin-treated normoxic carotid bodies the cAMP level was close to control, but in pertussis toxin-treated hypoxic carotid...Continue Reading

References

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Citations

Oct 6, 2010·Age·Teresa Castro MonteiroEmília Carreira Monteiro
Aug 6, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Mikko Nikinmaa
Apr 19, 2012·Canadian Journal of Physiology and Pharmacology·Amy TseFrederick W Tse
Oct 23, 1997·The Journal of Physiology·E Carpenter, C Peers
Nov 13, 2014·Frontiers in Physiology·Ana R NunesEmília C Monteiro
Mar 5, 2016·The Journal of Physiology·Ting ZhouHiroaki Matsunami
Nov 11, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·L HeC Gonzalez
Jul 8, 2009·The Journal of Physiology·Asuncion RocherConstancio Gonzalez
May 24, 2017·Frontiers in Physiology·Elvira Gonzalez-ObesoAngela Gomez-Niño

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