PMID: 8469928Apr 1, 1993Paper

Cholera toxin enhances alloantigen presentation by cultured intestinal epithelial cells

Scandinavian Journal of Immunology
A K BromanderN Y Lycke

Abstract

In the present study we show that cholera toxin (CT) strongly potentiates antigen presentation by intestinal epithelial cells, probably by enhancing co-stimulation. This was demonstrated in an allogeneic system using cells from the IEC-17 rat epithelial cell line as antigen presenting cells (APC). These cells were induced by optimal concentrations of IFN-gamma to express good amounts of Ia antigen and cultured for 24-48 h in the presence or absence of CT. Thereafter the cells were thoroughly washed and added to cultures containing MHC-incompatible spleen cells as responder cells. Epithelial cells exposed to CT demonstrated greatly enhanced ability to trigger allogen-specific T-cell proliferation as compared with IEC-17 cells treated with IFN-gamma alone. The mechanism for the enhanced APC function was investigated by analysing CT-treated IEC-17 cells for increased class II MHC antigen expression or enhanced production of cytokines with known co-stimulatory function. We found no significant increase in class II MHC antigen expression. By contrast, CT strongly promoted, in a dose-dependent fashion, the production of both IL-1 and IL-6 cytokines by IEC-17 cells as compared with untreated epithelial cells. This effect of CT was spe...Continue Reading

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Citations

Oct 9, 2002·Toxicon : Official Journal of the International Society on Toxinology·C F G VianaR A Ribeiro
Oct 3, 1999·Veterinary Immunology and Immunopathology·P Quéré, F Girard
Aug 23, 2001·Advanced Drug Delivery Reviews·P N Boyaka, J R McGhee
May 20, 1998·Research in Immunology·N Lycke
Apr 7, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Richard S BlumbergSean P Colgan
Jun 27, 2013·Infection and Immunity·Jessica Queen, Karla J F Satchell
Feb 20, 2007·Infection and Immunity·Carl-Fredrik FlachJan Holmgren
Jun 19, 2004·Molecular Membrane Biology·Lolke De Haan, Timothy R Hirst
Mar 1, 2005·Vaccine·L C Freytag, J D Clements
Sep 1, 1993·European Journal of Immunology·E Hörnquist, N Lycke
Oct 19, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alexandra DuvergerProsper N Boyaka
May 1, 1995·Clinical and Experimental Immunology·A PanjaL Mayer
Dec 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J W LillardJ R McGhee
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·H F StaatsB F Haynes
Jan 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alexandra DuvergerProsper N Boyaka
Feb 13, 1996·Annals of the New York Academy of Sciences·A Khoruts, M K Jenkins
Jan 11, 2020·Frontiers in Immunology·Monica C GestalEric T Harvill

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