Cholera toxin-specific memory B cell responses are induced in patients with dehydrating diarrhea caused by Vibrio cholerae O1.

The Journal of Infectious Diseases
Channa R JayasekeraS B Calderwood

Abstract

Infection with Vibrio cholerae induces durable immunity against subsequent disease, a process hypothesized to reflect anamnestic immune responses at the intestinal mucosa. The presence of antigen-specific memory B cells may therefore be a more direct measure of protection than serum antibody responses. We measured immunoglobulin (Ig) G memory B cells specific to cholera toxin B subunit (CTB) in 14 patients up to 90 days after V. cholerae O1 infection, by polyclonal stimulation of peripheral blood mononuclear cells followed by standard enzyme-linked immunospot assay. All patients generated CTB-specific IgG memory B cell responses by day 30 (mean, 0.10% of total circulating IgG memory B cells; range, 0.037%-0.28%), which persisted to day 90 (mean, 0.07%; range, 0.003%-0.27%). In contrast, circulating CTB-specific IgG antibody-secreting cells and serum vibriocidal and anti-CTB antibody responses peaked on day 7 and declined to undetectable or significantly lower levels by day 90. CTB-specific IgG memory B cell responses are detectable in the circulation at least 3 months after V. cholerae O1 infection and remain measurable even after serum antibody titers have declined to undetectable or considerably lower levels. This suggests th...Continue Reading

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Citations

Jun 17, 2009·Infection and Immunity·Aaron M HarrisJason B Harris
Aug 26, 2009·Infection and Immunity·Ana A WeilStephen B Calderwood
Feb 25, 2009·Infection and Immunity·Taufiqur Rahman BhuiyanFirdausi Qadri
May 3, 2014·PLoS Neglected Tropical Diseases·Richelle C CharlesJason B Harris
Apr 1, 2010·Toxins·Susana CubillosKatja Lehmann
Jun 20, 2012·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Karina RamirezMarcela F Pasetti
May 8, 2013·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Rezwanul WahidMarcelo B Sztein
Feb 24, 2012·Gut Microbes·Kathrin ZimmermannAnnette Oxenius
Sep 30, 2017·Cells·Josué da Costa Lima-JuniorFátima Conceição-Silva
Sep 21, 2012·Clinical and Vaccine Immunology : CVI·Marcela F Pasetti, Myron M Levine
Dec 3, 2009·Current Opinion in Gastroenterology·Jakub Simon, Karen Kotloff
May 19, 2011·Global Public Health·Hani Mowafi
Jun 17, 2016·PLoS Neglected Tropical Diseases·Wilfredo R MatiasJason B Harris
Oct 13, 2020·Frontiers in Immunology·Souwelimatou Amadou Amani, Mark L Lang
May 21, 2021·PLoS Neglected Tropical Diseases·Tiffany Leung, Laura Matrajt

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