PMID: 2508632Sep 1, 1989Paper

Cholera toxin treatment produces down-regulation of the alpha-subunit of the stimulatory guanine-nucleotide-binding protein (Gs)

The Biochemical Journal
G MilliganM J Wakelam

Abstract

The effect of activation of the alpha-subunit(s) of the stimulatory guanine-nucleotide-binding protein, Gs, on levels of this polypeptide(s) associated with the plasma membrane of L6 skeletal myoblasts was ascertained. Incubation of these cells with cholera toxin led to a time- and concentration-dependent 'down-regulation' of both 44 and 42 kDa forms of Gs alpha as assessed by immunoblotting with an anti-peptide antiserum (CS1) able to identify the extreme C-terminus of Gs. The effect of cholera toxin was specific for Gs; levels of Gi alpha in membranes of cholera toxin-treated cells were not different from untreated cells. Down-regulation of Gs was absolutely dependent upon prior ADP-ribosylation, and hence activation of Gs and was not mimicked by other agents which elevate intracellular levels of cyclic AMP. Pretreatment with pertussis toxin, which catalyses ADP-ribosylation of Gi but not of Gs, did not down-regulate either Gi or Gs, demonstrating that covalent modification by ADP-ribosylation is alone not a signal for removal of G-proteins from the plasma membrane.

Citations

Dec 12, 2001·American Journal of Physiology. Endocrinology and Metabolism·Ricky Cheung, Jane Mitchell
Oct 1, 1992·Journal of Neurochemistry·T Babila, D C Klein
Dec 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M RansjöO Ljunggren
Jan 1, 1995·Journal of Cancer Research and Clinical Oncology·V N GorelovP E Goretzki
Jan 28, 2014·PloS One·Anna A PimenovaIna Tesseur
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Jul 14, 1995·The Journal of Biological Chemistry·T M PalmerG L Stiles
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Jan 10, 2002·Cellular Signalling·Markus KlingerChristian Nanoff
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Jul 1, 1993·Cellular Signalling·N J Pyne, S Pyne
Jun 1, 1991·Trends in Pharmacological Sciences·G Milligan, A Green

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