Cholestatic liver damage is mediated by lymphocyte function antigen-1-dependent recruitment of leukocytes

Surgery
S DoldHenrik Thorlacius

Abstract

The role of specific adhesion molecules in cholestasis-induced leukocyte recruitment in the liver is not known. Therefore, the aim of our experimental study was to evaluate the role of lymphocyte function antigen-1 (LFA-1) in cholestatic liver injury. C57BL/6 mice underwent bile duct ligation for 12 hours. Mice were pretreated with an anti-LFA-1 antibody or control antibody. Subsequently, hepatic accumulation of leukocytes and sinusoidal perfusion were determined by means of intravital fluorescence microscopy. Hepatocellular damage was monitored by measuring serum levels of alanine aminotransferase and aspartate aminotransferase. CXC chemokines in the liver were determined by enzyme-linked immunosorbent assay. Bile duct ligation provoked clear-cut recruitment of leukocytes and liver damage, as indicated by increased serum activities of liver enzymes and sinusoidal perfusion failure. Neutrophils expressed greater levels of LFA-1 and inhibition of LFA-1 significantly decreased serum activity of alanine aminotransferase and aspartate aminotransferase levels in cholestatic mice. Immunoneutralization of LFA-1 reduced leukocyte adhesion in postsinusoidal venules that had been induced by bile duct ligation, whereas leukocyte rolling a...Continue Reading

References

Jun 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M H BemelmansW A Buurman
Sep 1, 1990·The Journal of Experimental Medicine·P Tekamp-OlsonA Cerami
Oct 1, 1995·Clinical Immunology and Immunopathology·R H Swanborg
Apr 1, 1994·Transfusion Medicine Reviews·H Uchiyama, K C Anderson
Oct 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T KimuraM Fujiwara
Feb 13, 1999·Critical Reviews in Immunology·A ZlotnikJ A Hedrick
Jul 29, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jaspreet S GujralHartmut Jaeschke
Oct 18, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jaspreet S GujralHartmut Jaeschke
Jan 13, 2004·Clinical and Diagnostic Laboratory Immunology·Daniel KlintmanHenrik Thorlacius
Jan 28, 2004·British Journal of Pharmacology·Xiang LiHenrik Thorlacius
Aug 28, 2004·Journal of Leukocyte Biology·Hartmut Jaeschke, Mary Lynn Bajt
Dec 15, 2004·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·R SchrammH Thorlacius
Jan 27, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Matthias W LaschkeHenrik Thorlacius
Jan 16, 2008·The British Journal of Surgery·P GeorgievP-A Clavien

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Citations

Oct 11, 2012·World Journal of Gastroenterology : WJG·Benjamin L Woolbright, Hartmut Jaeschke
Oct 15, 2009·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Stefan DoldHenrik Thorlacius
Jun 6, 2009·Transplant International : Official Journal of the European Society for Organ Transplantation·Yuan-Xing LiuShu-Sen Zheng
Jan 20, 2011·British Journal of Pharmacology·Darbaz AwlaHenrik Thorlacius
Jun 11, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Eleonora Patsenker, Felix Stickel
Jun 25, 2019·Frontiers in Immunology·Heidi HarjunpääSusanna C Fagerholm

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