Cholesteatoma-associated pathogenicity: potential role of lysosomal exoglycosidases

Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology
Ewa OlszewskaAndrzej Sieskiewicz

Abstract

The enzymatic profile of lysosomal exoglycosidases in middle ear cholesteatoma has not been well known. The assessment of glycoconjugate catabolism may contribute to a better understanding of cholesteatoma pathogenesis. The study aim was to evaluate catabolic processes of glycoproteins, glycolipids, and proteoglycans in cholesteatoma through outlining the concentration of N-acetyl-β-hexosaminidase (HEX), β-glucuronidase (GLUC), and β-galactosidase (GAL) activity as well as in serum of cholesteatoma patients and healthy volunteers. Acquired cholesteatomas (n = 25) and normal retroauricular skin specimens (n = 25) were taken during surgery as well as serum from cholesteatoma patients and healthy volunteers. HEX, GAL, and GLUC activity was assessed on basis of p-nitrophenol release from derivatives of the substrate (HEX: N-acetylglucosamine i N-acetylgalactosamine, GAL from galactose, and GLUC from glucuronide). The mean concentration of activity of HEX 1142.39 pKat/ml, GAL 8.90 pKat/ml, and GLUC 14.06 pKat/ml was significantly higher compared with the concentration of enzyme activity in normal tissue: HEX 267.65 pKat/ml, GAL 3.44 pKat/ml, and GLUC 3.90 pKat/ml. In the serum of cholesteatoma patients, the mean concentration of enz...Continue Reading

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Citations

Oct 23, 2015·Proceedings of the National Academy of Sciences of the United States of America·Won Ho YangJamey D Marth
Jul 22, 2016·Clinical and Experimental Otorhinolaryngology·Mahmood A HamedKenji Suzuki
Dec 17, 2016·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Shumin XieWei Liu
Jan 12, 2017·Clinical and Experimental Otorhinolaryngology·Mahmood A HamedYutaka Tsutsumi

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