PMID: 11342149May 9, 2001Paper

Cholesterol attenuates the interaction of the antimicrobial peptide gramicidin S with phospholipid bilayer membranes

Biochimica Et Biophysica Acta
E J PrennerRonald N McElhaney

Abstract

We have investigated the effect of the presence of 25 mol percent cholesterol on the interactions of the antimicrobial peptide gramicidin S (GS) with phosphatidylcholine and phosphatidylethanolamine model membrane systems using a variety of methods. Our circular dichroism spectroscopic measurements indicate that the incorporation of cholesterol into egg phosphatidylcholine vesicles has no significant effect on the conformation of the GS molecule but that this peptide resides in a range of intermediate polarity as compared to aqueous solution or an organic solvent. Our Fourier transform infrared spectroscopic measurements confirm these findings and demonstrate that in both cholesterol-containing and cholesterol-free dimyristoylphosphatidylcholine liquid-crystalline bilayers, GS is located in a region of intermediate polarity at the polar--nonpolar interfacial region of the lipid bilayer. However, GS appears to be located in a more polar environment nearer the bilayer surface when cholesterol is present. Our (31)P-nuclear magnetic resonance studies demonstrate that the presence of cholesterol markedly reduces the tendency of GS to induce the formation of inverted nonlamellar phases in model membranes composed of an unsaturated ph...Continue Reading

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Citations

Feb 11, 2005·The Journal of Membrane Biology·E J Westover, D F Covey
Oct 20, 2006·European Biophysics Journal : EBJ·Heiko Heerklotz, Joachim Seelig
Nov 12, 2002·Chemistry and Physics of Lipids·Marie Paule Mingeot-LeclercqRobert Brasseur
Dec 17, 2008·Quarterly Reviews of Biophysics·Heiko Heerklotz
Nov 16, 2001·Antimicrobial Agents and Chemotherapy·M P Mingeot-LeclercqR Brasseur
Jul 25, 2008·Future Microbiology·Dirk Kraus, Andreas Peschel
Mar 10, 2012·Journal of Invertebrate Pathology·Daniela A Moreno-HabelEric J Haas-Stapleton
Aug 31, 2007·Bioorganic & Medicinal Chemistry·Michael A MarquesClay C Wang
Jul 15, 2009·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Emilios Andrew PapanastasiouBarney Michael Bishop
Sep 8, 2012·Microbiology·Aline Dias PaivaHilário Cuquetto Mantovani
Jul 24, 2015·ACS Applied Materials & Interfaces·Cuixia ChenJian Ren Lu
May 7, 2011·Biochimica Et Biophysica Acta·Hiren PatelHeiko Heerklotz
Aug 14, 2012·Biochimica Et Biophysica Acta·Amy WonAnatoli Ianoul
May 17, 2005·Biochimica Et Biophysica Acta·Regine WillumeitJörg Andrä
Sep 15, 2004·FEMS Immunology and Medical Microbiology·Mónica Viejo-DíazJosé F Fierro
Jan 10, 2017·ACS Chemical Biology·Mintu PorelChristopher A Alabi
Sep 22, 2018·Macromolecular Bioscience·Emily A HoffChristopher A Alabi
Dec 15, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A James MasonBurkhard Bechinger
Jun 26, 2002·The Journal of Peptide Research : Official Journal of the American Peptide Society·M Jelokhani-NiarakiR S Hodges
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Dec 4, 2020·International Journal of Molecular Sciences·Marcin MakowskiSónia Gonçalves
Oct 19, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Meirav Apel-PazT Kyle Vanderlick
Mar 8, 2011·Journal of Chemical Theory and Computation·Emma S E Eriksson, Leif A Eriksson
May 12, 2018·ACS Infectious Diseases·Christine M ArtimChristopher A Alabi

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