Cholesterol-based tethers and markers for model membranes investigation

Chemistry and Physics of Lipids
O Eicher-LorkaGintaras Valincius

Abstract

A series of new bifunctional cholesterol compounds for tethered bilayer membrane (tBLM) systems were synthesized and tested. The compounds containing cyclic disulfide group may be used as molecular anchors for phospholipid bilayers. Anchoring occurs through the insertion of the cholesterol moiety into the hydrophobic slab of the phospholipid layer, while the surface density of anchor molecules may be adjusted using disulfides terminated spacers. Five ethylene oxide segments between the disulfide group and the cholesteryl provide hydration of the layer separating solid support and model membrane. Another group of cholesterol derivatives described in this work contains either fluorescence probe or electroactive functional groups. We demonstrated the practical utility of these compounds for visualization of cholesterol extraction from and loading to tBLMs. We demonstrated that electroactive group containing cholesterol derivatives can be reconstituted either into vesicles or tBLMs. In both cases an electrochemical signal can be generated on electrodes from these probes. In general, the newly synthesized compound may be utilized in a variety of applications involving tethered bilayer systems and vesicles.

References

May 11, 1999·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·A ZimmerC R Noe
Nov 6, 2001·Progress in Lipid Research·Henna Ohvo-RekiläJ Peter Slotte
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Julia J HwangSamuel I Stupp
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·Joost C M Holthuis, Tim P Levine
May 26, 2006·The Journal of Physical Chemistry. B·Gintaras ValinciusMathias Lösche
Jan 24, 2007·Chemistry and Physics of Lipids·Daniel Wüstner
Jul 6, 2007·European Biophysics Journal : EBJ·Claire Rossi, Joël Chopineau
Nov 3, 2007·Current Opinion in Chemical Biology·Yee-Hung M Chan, Steven G Boxer
Aug 29, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Frank HeinrichMathias Lösche
Dec 23, 2009·Biochimica Et Biophysica Acta·Daniel M Carter RamirezLinda J Johnston
Dec 1, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Gintaras ValinciusFeliksas Ivanauskas
Jan 24, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Christian KleuschAgnes Csiszár
May 23, 2012·Colloids and Surfaces. B, Biointerfaces·Tao ZhuYuqiang Ma
Sep 27, 2012·Sensors·Young-Rok KimTae-Joon Jeon
Jun 12, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Rima BudvytyteDavid J Vanderah
Sep 21, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Yuri GerelliGiovanna Fragneto
Dec 24, 2013·Chemistry & Biology·Andrey S Klymchenko, Rémy Kreder
Dec 3, 2014·Chemistry and Physics of Lipids·Tomasz Róg, Ilpo Vattulainen
Dec 20, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Bozena RakovskaGintaras Valincius
Jan 1, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Giulio PretaI Martin Sheldon

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Citations

May 24, 2016·Biochimica Et Biophysica Acta·Giulio PretaGintaras Valincius
Mar 3, 2020·Biochimica Et Biophysica Acta. Biomembranes·Tomas SabirovasGintaras Valincius

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