Cholesterol-dependent interaction of polyunsaturated phospholipids with Na,K-ATPase

Biochemistry
Flemming Cornelius

Abstract

Polyunsaturated phospholipids such as 16:0-22:6 PC and 22:6 PC both stabilized the E1 conformation and inhibited turnover of Na,K-ATPase reconstituted into 18:1 PC or 18:1 PC/cholesterol liposomes. The inhibition increases in the order 22:6 PC > 16:0-22:6 PC both in the presence and in the absence of cholesterol, but is most pronounced in the absence of cholesterol. The inhibition of Na,K-ATPase turnover may thus correlate with the capability of polyunsaturated phospholipids and cholesterol to induce liquid-disordered and liquid-ordered lipid phases, respectively. In the presence of cholesterol 16:0-22:6 PC and 22:6 PC both increase the apparent Na+ affinity and change the K+ inhibition observed at low ATP concentration into activation. These effects on Na,K-ATPase kinetics can be explained by the ability of polyunsaturated phospholipids to induce lateral phase separation from cholesterol, which may be partially excluded from interaction with the Na,K-ATPase/lipid interface. Finally, inclusion of polyunsaturated phospholipids may induce changes in the bilayer hydrophobic thickness, which will increase the hydrophobic mismatch between lipids and protein.

References

Mar 6, 1981·Biochimica Et Biophysica Acta·A JohannssonJ C Metcalfe
Jan 1, 1993·Annual Review of Biophysics and Biomolecular Structure·O G Mouritsen, M Bloom
May 8, 1999·Biophysical Journal·R S Cantor
Sep 22, 2001·Biophysical Journal·C LüpfertR J Clarke
Dec 26, 2001·Biophysical Journal·Michael R BrzustowiczStephen R Wassall
Jul 12, 2002·The Journal of Biological Chemistry·Laura SegallRhoda Blostein
Oct 29, 2003·Chemistry and Physics of Lipids·William Stillwell, Stephen R Wassall
Jun 11, 2004·Biophysical Journal·Justin Gullingsrud, Klaus Schulten
Nov 3, 2004·Biochimica Et Biophysica Acta·Morten Ø Jensen, Ole G Mouritsen
Jul 31, 2007·Biophysical Journal·Arun Yethiraj, James C Weisshaar

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Citations

Oct 16, 2009·Molecular Interventions·Christopher B Divito, Susan G Amara
Jun 10, 2011·The Journal of Experimental Biology·Gaddiel Galarza-MuñozJoshua J C Rosenthal
Jul 9, 2013·Journal of Inorganic Biochemistry·V S SilvaP P Gonçalves
Feb 20, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Y ItokazuM Vornanen
Feb 23, 2013·The Journal of Biological Chemistry·Haim HavivSteven J D Karlish
Oct 7, 2014·Archives of Biochemistry and Biophysics·Juliana Sakamoto YonedaPietro Ciancaglini
Mar 21, 2016·Biochimica Et Biophysica Acta·Tripta BhatiaOle G Mouritsen
Oct 22, 2015·Biophysical Journal·Ronald J Clarke
Mar 21, 2015·Biochimica Et Biophysica Acta·F CorneliusS J D Karlish
Jan 22, 2013·Archives of Biochemistry and Biophysics·Juliana Sakamoto YonedaPietro Ciancaglini
Jul 27, 2012·Epilepsy & Behavior : E&B·Antônio-Carlos G De AlmeidaFulvio A Scorza
Jun 3, 2016·Frontiers in Physiology·Vladimir V Matchkov, Igor I Krivoi
Mar 10, 2017·American Journal of Physiology. Cell Physiology·Alexey M PetrovIgor I Krivoi
Feb 17, 2019·The Journal of Biological Chemistry·Alvaro GarciaRonald J Clarke
Oct 1, 2020·American Journal of Physiology. Cell Physiology·Jue ZhangYiliang Chen
May 11, 2019·Biophysics Reviews·Khondker R Hossain, Ronald J Clarke
Dec 24, 2014·The Journal of Biological Chemistry·Michael HabeckSteven J D Karlish
Mar 1, 2017·Proceedings of the National Academy of Sciences of the United States of America·Michael HabeckSteven J D Karlish
Jun 28, 2018·Frontiers in Physiology·María E Riveros, Mauricio A Retamal
Aug 30, 2019·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Elie FarhatJean-Michel Weber
Jan 17, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yu-Ting LinTsung-Han Lee

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