Cholesterol Depletion Regulates Axonal Growth and Enhances Central and Peripheral Nerve Regeneration

Frontiers in Cellular Neuroscience
Cristina Roselló-BusquetsEduardo Soriano

Abstract

Axonal growth during normal development and axonal regeneration rely on the action of many receptor signaling systems and complexes, most of them located in specialized raft membrane microdomains with a precise lipid composition. Cholesterol is a component of membrane rafts and the integrity of these structures depends on the concentrations present of this compound. Here we explored the effect of cholesterol depletion in both developing neurons and regenerating axons. First, we show that cholesterol depletion in vitro in developing neurons from the central and peripheral nervous systems increases the size of growth cones, the density of filopodium-like structures and the number of neurite branching points. Next, we demonstrate that cholesterol depletion enhances axonal regeneration after axotomy in vitro both in a microfluidic system using dissociated hippocampal neurons and in a slice-coculture organotypic model of axotomy and regeneration. Finally, using axotomy experiments in the sciatic nerve, we also show that cholesterol depletion favors axonal regeneration in vivo. Importantly, the enhanced regeneration observed in peripheral axons also correlated with earlier electrophysiological responses, thereby indicating functional...Continue Reading

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Citations

Jun 20, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jinyoung LeeYongcheol Cho
Apr 23, 2020·Frontiers in Molecular Neuroscience·Cristina Roselló-BusquetsRamon Martínez-Mármol
Oct 1, 2020·Nature Communications·Oshri AvrahamValeria Cavalli
Oct 24, 2020·Cellular and Molecular Life Sciences : CMLS·Marc Hernaiz-LlorensEduardo Soriano
Jan 13, 2021·Neurobiology of Disease·Alireza P ShabanzadehPhilippe P Monnier
Mar 6, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Narjes ShahsavaniSoheila Karimi-Abdolrezaee
Jun 3, 2021·International Journal of Molecular Sciences·Marc Hernaiz-LlorensEduardo Soriano
Jul 17, 2021·Neural Regeneration Research·Bor Luen Tang
Nov 9, 2021·Frontiers in Molecular Neuroscience·Mini JoseChaitra Channakeshava

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