Cholesterol does not induce segregation of liquid-ordered domains in bilayers modeling the inner leaflet of the plasma membrane

Biophysical Journal
T Y Wang, J R Silvius

Abstract

A fluorescence-quenching method has been used to assess the potential formation of segregated liquid-ordered domains in lipid bilayers combining cholesterol with mixtures of amino and choline phospholipids like those found in the cytoplasmic leaflet of the mammalian cell plasma membrane. When present in proportions >20-30 mol %, different saturated phospholipids show a strong proclivity to form segregated domains when combined with unsaturated phospholipids and cholesterol, in a manner that is only weakly affected by the nature of the phospholipid headgroups. By contrast, mixtures containing purely unsaturated phospholipids and cholesterol do not exhibit detectable segregation of domains, even in systems whose components differ in headgroup structure, mono- versus polyunsaturation and/or acyl chain heterogeneity. These results indicate that mixtures of phospholipids resembling those found in the inner leaflet of the plasma membrane do not spontaneously form segregated liquid-ordered domains. Instead, our findings suggest that factors extrinsic to the inner-monolayer lipids themselves (e.g., transbilayer penetration of long sphingolipid acyl chains) would be essential to confer a distinctive, more highly ordered organization to ...Continue Reading

References

May 17, 1979·Biochimica Et Biophysica Acta·Y NakagawaS Nojima
Oct 5, 1979·Biochimica Et Biophysica Acta·Y LangeD M Small
Jul 20, 1977·Biochimica Et Biophysica Acta·P Comfurius, R F Zwaal
Feb 23, 1978·Nature·C F SchmidtT E Thompson
Feb 14, 1977·Biochimica Et Biophysica Acta·R A DemelL L van Deenen
Jan 30, 1991·Biochimica Et Biophysica Acta·R C MacDonaldL R Hu
Sep 1, 1986·The Journal of Physiology·F J Alvarez-LeefmansH González-Serratos
Jan 1, 1989·Annual Review of Cell Biology·G van Meer
Jul 1, 1974·Lipids·R R Lowry, I J Tinsley
Sep 1, 1984·The Journal of Physiology·F J Alvarez-LeefmansT J Rink
Jul 1, 1994·The Journal of Cell Biology·A M Shenoy-ScariaD M Lublin
Jun 5, 1997·Nature·K Simons, E Ikonen
Jun 1, 1997·Molecular Biology of the Cell·A WolvenM D Resh
Nov 21, 1997·Biochemical and Biophysical Research Communications·D A Brown, E London
Jul 15, 1998·The Journal of Membrane Biology·D A Brown, E London
Jan 19, 1999·Annual Review of Cell and Developmental Biology·D A Brown, E London
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·J KorlachG W Feigenson
Aug 17, 1999·Current Opinion in Cell Biology·T V Kurzchalia, R G Parton

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Citations

May 25, 2012·Cell Biochemistry and Biophysics·Paola A CorsettoAngela M Rizzo
Jun 1, 2005·Cellular Signalling·Alexandre K Rouquette-JazdanianClaude Aussel
Nov 19, 2003·Cell·Sean Munro
Oct 18, 2002·Current Opinion in Cell Biology·Frederick R Maxfield
Aug 7, 2002·Current Opinion in Structural Biology·Erwin London
Feb 3, 2007·Biochemistry·Yvonne LangeTheodore L Steck
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Gerrit van MeerGerald W Feigenson
Jan 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Marcus D Collins, Sarah L Keller
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Robert A DickVolker M Vogt
Jul 16, 2004·The European Physical Journal. E, Soft Matter·E Schäffer, U Thiele
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Sushmita Mukherjee, Frederick R Maxfield
Jan 17, 2013·The Journal of Chemical Physics·Chloe M FunkhouserK Thornton
May 9, 2015·Membranes·Drew MarquardtGeorg Pabst
Mar 20, 2004·Traffic·Philippe F Devaux, Roger Morris
Feb 16, 2011·Biophysical Journal·G Garbès PutzelM Schick
Mar 27, 2016·Chemistry and Physics of Lipids·Daniel WüstnerMichael A Lomholt
Sep 20, 2008·Biochimica Et Biophysica Acta·Salvatore ChiantiaPetra Schwille
Jun 12, 2013·Biophysical Journal·Amit ChoubeyPriya Vashishta
Nov 5, 2002·Biophysical Journal·H Heerklotz
Nov 18, 2003·Traffic·Robert G Parton, Ayanthi A Richards
Apr 14, 2016·Biophysical Journal·Oskar EngbergJ Peter Slotte
Sep 20, 2012·Virus Research·A KervielD Muriaux
Sep 21, 2013·Biophysical Journal·Roie Shlomovitz, M Schick
Oct 2, 2007·Biophysical Journal·G Garbès Putzel, M Schick
Apr 18, 2013·Biochimica Et Biophysica Acta·M SchwieringN Hellmann
Sep 4, 2007·Biophysical Journal·Alexander J WagnerSylvio May
Aug 22, 2006·Biophysical Journal·Piet H M LommerseThomas Schmidt
Nov 12, 2013·Biophysical Journal·Lukasz M SolankoDaniel Wüstner
Oct 3, 2009·Biochimica Et Biophysica Acta·Marie-Cécile GiocondiChristian Le Grimellec
Oct 14, 2008·Biochimica Et Biophysica Acta·Volker KiesslingLukas K Tamm
Jun 26, 2013·Biophysical Journal·Matthew C BlosserSarah L Keller
Aug 26, 2004·Biochimica Et Biophysica Acta·P H M LommerseT Schmidt

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