Abstract
The objective of this study was to characterize the transfer of cholesterol from Manduca sexta larvae fat body to high-density lipophorin. [(3)H]-Cholesterol-labeled fat body was incubated with lipophorin under different conditions and cholesterol transfer was determined. Transfer rate exhibited a hyperbolic dependency on lipophorin concentration with an apparent K(m) of 3.6 mg/ml, which is consistent with either an aqueous diffusion mechanism of cholesterol transfer or a receptor-mediated process. Several results, including the high K(m), the high activation energy, and the lack of Ca(2+) dependence favor aqueous diffusion model. In addition, anti-lipid transfer particle antibodies had only a small inhibitory effect, suggesting it is not involved in cholesterol transfer. However, the transfer was inhibited in the presence of suramin, which would be consistent with a receptor-mediated process. The effects of suramin may be complex because it can change membrane properties when bound to the lipophorin receptor and affect the rate of cholesterol desorption. The preponderance of data suggests that the export of cholesterol from fat body to lipophorin follows a simple aqueous diffusion pathway. Although we cannot completely exclude...Continue Reading
References
Jun 15, 1978·Analytical Biochemistry·M A MarkwellN E Tolbert
Oct 1, 1991·Biochimica Et Biophysica Acta·W J JohnsonM C Phillips
Jan 1, 1988·Tissue & Cell·E WillottM A Wells
Jan 1, 1985·Progress in Lipid Research·A M BeenakkersW J Van Marrewijk
Aug 15, 1970·Nature·U K Laemmli
Mar 17, 1995·The Journal of Biological Chemistry·W S DavidsonM C Phillips
Jan 1, 1994·Advances in Protein Chemistry·J L Soulages, M A Wells
Oct 1, 1993·Insect Biochemistry and Molecular Biology·M C Van Heusden
Jan 1, 1996·Archives of Insect Biochemistry and Physiology·M C Van HeusdenJ H Law
Sep 1, 1995·Atherosclerosis·Z E JouniD J McNamara
Jul 5, 1996·The Journal of Biological Chemistry·P G YanceyG H Rothblat
Jun 4, 1996·Biochemistry·Y Zhao, Y L Marcel
Apr 4, 2000·Insect Biochemistry and Molecular Biology·K C Gondim, M A Wells
Aug 10, 2000·The Journal of Biological Chemistry·M FukasawaK Hanada
Dec 5, 2000·Insect Biochemistry and Molecular Biology·E L ArreseM A Wells
May 30, 2001·Insect Biochemistry and Molecular Biology·L E Canavoso, M A Wells
Feb 22, 2002·Archives of Insect Biochemistry and Physiology·Zeina E JouniMichael A Wells
Dec 18, 2003·Journal of Lipid Research·Lilian E CanavosoMichael A Wells
Citations
Sep 6, 2002·Insect Biochemistry and Molecular Biology·Hwa Kyung YunMichael A Wells
Jan 22, 2003·Insect Biochemistry and Molecular Biology·Z E JouniK Tsuchida
Aug 28, 2012·Journal of Insect Physiology·Xin DuHuazu Hong
Nov 3, 2004·Journal of Insect Physiology·Kozo TsuchidaZeina E Jouni
Jun 2, 2005·Insect Molecular Biology·E J BlitzerQ Lan
Oct 11, 2019·Annual Review of Entomology·Xiangfeng Jing, Spencer T Behmer
Apr 20, 2021·Frontiers in Physiology·Petter F EntringerKatia C Gondim
Jul 20, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Zeina E JouniMichael A Wells