Cholesterol enhances phospholipid binding and aggregation of annexins by their core domain

Biochemical and Biophysical Research Communications
Jesus Ayala-Sanmartin

Abstract

Annexins are Ca(2+)-dependent phospholipid-binding proteins composed of two domains: A conserved core that is responsible for Ca(2+)- and phospholipid-binding, and a variable N-terminal tail. A Ca(2+)-independent annexin 2-membrane association has been shown to be modulated by the presence of cholesterol in the membranes. Herein, the roles of the core and the N-terminal tail on the cholesterol-enhancement of annexin 2 membrane binding and aggregation were studied. The results show that (i) the cholesterol-mediated increase in membrane binding and in the Ca(2+) sensitivity for membrane aggregation were not modified by a N-terminal peptide (residues 15-26), and were conserved in mutants of the N-terminal end (S11 and S25 substitutions); (ii) cholesterol induced an increase in the Ca(2+)-dependent membrane binding and aggregation of the N-terminally truncated protein (Delta 1-29); and (iii) annexins 5 and 6, two proteins with unrelated N-terminal tails and homologous core domains showed a cholesterol-mediated enhancement of the Ca(2+)-dependent binding to membranes. These data indicate that the core domain is responsible for the cholesterol-mediated effects. A model for the cholesterol effect in membrane organisation, annexin bind...Continue Reading

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Citations

Nov 1, 2011·Molecular Biology of the Cell·Meritxell ReverterCarlos Enrich
Apr 22, 2009·Cellular and Molecular Life Sciences : CMLS·Katia MonastyrskayaAnnette Draeger
Dec 14, 2011·Cellular and Molecular Life Sciences : CMLS·Magdalena DomonJoanna Bandorowicz-Pikula
Apr 7, 2010·Journal of Colloid and Interface Science·Magdalena M DomonFrançoise Besson
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Feb 9, 2016·Immunology and Cell Biology·Rhea CornelyKatharina Gaus
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Mar 4, 2014·Cellular Signalling·Monira HoqueThomas Grewal
Oct 5, 2010·Biochimica Et Biophysica Acta·Carlos EnrichThomas Grewal
May 18, 2010·Biochimica Et Biophysica Acta·Françoise IllienJesus Ayala-Sanmartin
Apr 30, 2002·Experimental Cell Research·Ariane-Valérie FaureJesus Ayala-Sanmartin
May 8, 2015·Journal of Lipids·Ravi Kant Upadhyay
Apr 20, 2002·Biophysical Journal·Aneta KirilenkoJoanna Bandorowicz-Pikula
Dec 11, 2007·Biochimica Et Biophysica Acta·Jesus Ayala-SanmartinJacques Gallay
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May 30, 2008·The Journal of Biological Chemistry·Malik ZiboucheJesus Ayala-Sanmartin
Jan 10, 2014·The Journal of Biological Chemistry·Priyam Banerjee, Arun Bandyopadhyay
Jan 1, 2004·The Journal of Biological Chemistry·Olivier LambertJesus Ayala-Sanmartin
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Mar 19, 2019·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Malik ZiboucheJesus Ayala-Sanmartin
Jan 24, 2012·Journal of Cell Science·Ghita GhislatErwin Knecht

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