Cholesterol in remnant-like lipoproteins in human serum using monoclonal anti apo B-100 and anti apo A-I immunoaffinity mixed gels

Clinica Chimica Acta; International Journal of Clinical Chemistry
K NakajimaE Campos

Abstract

We have developed a simple, rapid assay method for apo E-rich lipoproteins (d < 1.006 g/ml), using an immunoaffinity gel mixture of anti apo B-100 and apo A-I antibodies coupled to Sepharose 4B. The immunoaffinity mixed gels adsorb normal lipoproteins containing apo A-I quantitatively as well as most lipoproteins containing apo B-100. Unbound lipoproteins are quantified by assay of cholesterol. Characterization of the unbound lipoproteins of d < 1.006 g/ml (J Lipid Res 1992; 33: 369-380) has shown that they represent chylomicron and VLDL remnant-like particles (RLP). RLP-Cholesterol(C) levels in plasma have been determined in 363 male and female normolipidemic subjects (mean +/- S.D.: 72 +/- 16 mg/l) and have been found to be higher in patients with coronary heart disease and familial dysbetalipoproteinemia. Triglyceride-rich lipoproteins may well contain both atherogenic and non-atherogenic particles that can be separated by this simple immunoadsorption assay.

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Citations

Aug 20, 2002·The International Journal of Eating Disorders·Yasuhiko HommaKamon Iigaya
Mar 27, 2007·European Journal of Nutrition·Yuka KishimotoDennis T Gordon
Feb 25, 2005·Current Atherosclerosis Reports·ThB TwicklerJ S Cohn
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Dec 23, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Mitsuyo OkazakiKatsuyuki Nakajima
Apr 30, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Sanae TakeichiKatsuyuki Nakajima
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