Cholesterol-Loaded Cyclodextrin Increases the Cholesterol Content of Goat Sperm To Improve Cold- and Osmotic-Resistance and Maintain Sperm Function after Cryopreservation

Biology of Reproduction
Vianney M SalmonJanice L Bailey

Abstract

The success of semen cryopreservation depends on sperm membrane integrity and function after thawing. Cholesterol-loaded cyclodextrin (CLC) is used for in vitro incorporation of cholesterol to protect cells against cold temperatures. We hypothesized that CLC treatment also enhances sperm cholesterol content to increase tolerance to osmotic shock and cryoresistance, thereby improving fertility. We confirmed that treatment of goat semen with 3 mg/ml CLC increases sperm cholesterol content using both the Liebermann-Burchard approach and Filipin labelling of membrane cholesterol. Sperm were then treated ± CLC and cryopreserved. After thawing, sperm cholesterol dramatically fell even in the presence of CLC, which explains the mechanism of "cryocapacitation." CLC treatment, however, maintained a normal pre-freeze cholesterol level in sperm after cryopreservation. Furthermore, fresh sperm treated with CLC and subjected to either cold shock or incubated in hypo-, iso- and hyperosmotic media, designed to mimic stresses associated with freezing/thawing, displayed increased temperature and osmotic tolerance. CLC treatment also improved sperm viability, motility and acrosome integrity after thawing. Furthermore, the CLC treatment did not a...Continue Reading

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Citations

Apr 30, 2017·Molecular Reproduction and Development·Bart M Gadella
Dec 5, 2019·Biomolecules·Susana Santos Braga
Jun 3, 2020·Zygote : the Biology of Gametes and Early Embryos·Linlin SunWenxian Zeng
May 30, 2020·International Journal of Molecular Sciences·Marcia de Almeida Monteiro Melo FerrazNucharin Songsasen
Apr 23, 2020·International Journal of Molecular Sciences·Patricia Peris-FrauJ Julián Garde
Jun 12, 2019·Reproduction, Fertility, and Development·Lucía Martínez-FresnedaJulian Santiago-Moreno
Apr 19, 2021·Domestic Animal Endocrinology·V N Flores-GilJ Santiago-Moreno

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