Cholesterol-lowering drugs the simvastatin and atorvastatin change the protease activity of pepsin: An experimental and computational study.

International Journal of Biological Macromolecules
Mohsen ShahlaeiSajad Moradi

Abstract

In this study, the effect of long-term use drugs of cholesterol-lowering atorvastatin and simvastatin on the activity and molecular structure of pepsin as important gastric enzyme was investigated by various experimental and computational methods. Based on the results obtained from fluorescence experiments, both drugs can bond to pepsin and quench the fluorescence intensity of protein through the static quenching mechanism. Also analysis of the thermodynamic parameters of binding the drugs to pepsin showed that the main forces in the complex formation for both are hydrophobic interactions and van der Waals forces. The effects of the drugs on the enzymatic activity of pepsin were then investigated and results showed that in the presence of both drugs the catalytic activity of the enzyme was significantly increased in lower (0.3-0.6 mM) concentrations however about the atorvastatin, increasing the concentration (0.9 mM) decreased the protease activity of pepsin. Also as a result of the FTIR studies, it was found that binding of the drugs to protein did not significant alteration in the structure of the protein. In order to obtain the atomic details of drug-protein interactions, the computational calculations were performed. The r...Continue Reading

References

Mar 1, 1986·Gut·C W Venables
Jan 1, 1972·Methods in Enzymology·K WeberM Osborn
May 1, 1995·Protein Science : a Publication of the Protein Society·M FujinagaM N James
Jan 1, 1996·Journal of Molecular Recognition : JMR·D S GoodsellA J Olson
May 31, 2002·Acta Crystallographica. Section D, Biological Crystallography·Helen M BermanChristine Zardecki
Jun 18, 2002·Journal of Cellular and Molecular Medicine·C Stancu, A Sima
Jul 2, 2002·The Quarterly Review of Biology·Joseph S Fruton
Oct 1, 1959·Archives of Biochemistry and Biophysics·J CERBULISC A ZITTLE
Oct 23, 2004·Pharmaceutical Research·Jerome H HochmanThomayant Prueksaritanont
Feb 12, 2008·Journal of Pharmaceutical and Biomedical Analysis·Han-Na HouYi Liu
Dec 17, 2008·Current Protocols in Bioinformatics·Garrett M MorrisArthur J Olson
Aug 15, 2012·Journal of Cheminformatics·Marcus D HanwellGeoffrey R Hutchison
Mar 13, 2014·International Journal of Biological Macromolecules·Hua-jin ZengLing-bo Qu
Aug 1, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Liangliang ShenYinong Li
Nov 11, 2014·Molecular BioSystems·Sumit Kumar ChaturvediRizwan Hasan Khan
May 6, 2015·International Journal of Biological Macromolecules·Ming YingKai Zhou
Jul 15, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hua-Jin ZengLing-Bo Qu
Dec 13, 2011·Journal of Chemical Theory and Computation·Alpeshkumar K MaldeAlan E Mark
Mar 1, 2008·Journal of Chemical Theory and Computation·Berk HessErik Lindahl
Sep 2, 2016·PloS One·Panos ZirosIoannis G Habeos
Jul 12, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hrvoje RimacMirza Bojić
Jun 2, 2018·Research in Pharmaceutical Sciences·Rezvan Rezaee NasabFarshid Hassanzadeh
Nov 13, 2018·Biochimica Et Biophysica Acta. General Subjects·Anna Rita BizzarriSalvatore Cannistraro
Nov 27, 2018·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Zhezhu NanRunguang Sun
Dec 10, 2019·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Sajad MoradiMohsen Shahlaei

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Medical Clinics of North America
D F Magee
The American Journal of Dental Science
R T Edes
© 2021 Meta ULC. All rights reserved