Cholesterol metabolites alleviate injured liver function and decrease mortality in an LPS-induced mouse model

Metabolism: Clinical and Experimental
Yanxia NingShunlin Ren

Abstract

Oxysterol sulfation plays a fundamental role in the regulation of many biological events. Its products, 25-hydroxycholesterol 3-sulfate (25HC3S) and 25-hydroxycholesterol 3, 25-disulfate (25HCDS), have been demonstrated to be potent regulators of lipid metabolism, inflammatory response, cell apoptosis, and cell survival. In the present study, we tested these products' potential to treat LPS-induced acute liver failure in a mouse model. Acute liver failure mouse model was established by intravenous injection with LPS. The injured liver function was treated with intraperitoneal administration of 25HC, 25HC3S or 25HCDS. Serum enzymatic activities were determined in our clinic laboratory. ELISA assays were used to detect pro-inflammatory factor levels in sera. Western blot, Real-time Quantitative PCR and RT2Profiler PCR Array analysis were used to determine levels of gene expression. Administration of 25HC3S/25HCDS decreased serum liver-impaired markers; suppressed secretion of pro-inflammatory factors; alleviated liver, lung, and kidney injury; and subsequently increased the survival rate in the LPS-induced mouse model. These effects resulted from the inhibition of the expression of genes involved in the pro-inflammatory response ...Continue Reading

Citations

Dec 6, 2017·World Journal of Gastroenterology : WJG·Lei FengYi Gao
Mar 19, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pauline BottemanneGiulio G Muccioli
Apr 6, 2021·British Journal of Pharmacology·Owein Guillemot-Legris, Giulio G Muccioli
May 28, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Xiaohui WangQiang Meng
Sep 9, 2021·ACS Biomaterials Science & Engineering·Xun LiuKun Qian

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