Cholesterol modulates interaction between an amphipathic class A peptide, Ac-18A-NH2, and phosphatidylcholine bilayers

Biochemistry
Masashi EgashiraTetsurou Handa

Abstract

Cholesterol (Chol) in phosphatidylcholine large unilamellar vesicles (PC LUV) modulated interaction of the bilayers with a class A amphipathic peptide, Ac-18A-NH2: Chol increased the peptide binding capacity and reduced the affinity together with the peptide-induced leakage of calcein from LUV. Similar effects of Chol have been observed on the interaction of LUV with apoA-I [Saito, H., Miyako, Y., Handa, T., and Miyajima, K. (1997) J. Lipid Res. 38, 287-294]. Circular dichroism (CD) spectra of the peptide indicated a similar helical structure formation in LUV with and without Chol. The fluorescence spectral shift, quantum yield, anisotropy, and acrylamide-quenching of the peptide Trp indicated that in PC:Chol (3:2) LUV, Ac-18A-NH2 was located in a more polar membrane environment with increased motional freedom and greater accessibility to the aqueous medium. Fluorescence energy transfer from the Trp indole ring to acceptors situated at different depths in the bilayers revealed that the amphipathic peptide penetrated the hydrophobic interior of PC bilayers, while the peptide was located at the polar zwitterionic surface in PC:Chol LUV. The inclusion of Chol causes the headgroup separation of PC at the surface of LUV and increase...Continue Reading

Citations

Aug 25, 2009·Journal of Lipid Research·Shobini JayaramanOlga Gursky
Mar 12, 2010·Journal of Virology·Jeremy S RossmanRobert A Lamb
Jun 5, 2003·Biochimica Et Biophysica Acta·Mayumi YamamotoTetsurou Handa
May 13, 2006·Annual Review of Biophysics and Biomolecular Structure·Thomas J McIntosh, Sidney A Simon
Nov 6, 2007·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Masafumi Tanaka
Nov 24, 2011·Chemistry and Physics of Lipids·Yuki TakechiHiroyuki Saito
Dec 3, 2008·Journal of Peptide Science : an Official Publication of the European Peptide Society·Masafumi TanakaHiroyuki Saito
Jun 9, 2006·Archives of Biochemistry and Biophysics·Su Duy NguyenDai-Eun Sok
Mar 18, 2006·Colloids and Surfaces. B, Biointerfaces·Shaoqian LiuYuanjian Li
Sep 29, 2005·Analytical Biochemistry·W E MomsenH L Brockman
Sep 21, 2010·Cell·Jeremy S RossmanRobert A Lamb
Jun 21, 2016·Biochimica Et Biophysica Acta·Gi Young LeeYoung Do Yoo
Jun 4, 2019·Biological & Pharmaceutical Bulletin·Haruka KawaharaKeiichiro Okuhira
Dec 14, 2011·The Journal of Physical Chemistry. B·Mai OzawaMinoru Nakano
May 29, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Minh D PhanKwanwoo Shin
Jul 10, 2020·The Journal of Physical Chemistry. B·Agnieszka MartynaJeremy S Rossman
May 18, 2020·Biochimica Et Biophysica Acta. Biomembranes·Shipeng HuZhihua Sun

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Frédérick de Meyer, Berend Smit
Proceedings of the National Academy of Sciences of the United States of America
Ming-Tao LeeHuey W Huang
Current Opinion in Cell Biology
Bruno Antonny
© 2022 Meta ULC. All rights reserved