PMID: 3768382Oct 24, 1986Paper

Cholesterol-phospholipid vesicles in human bile: an ultrastructural study

Biochimica Et Biophysica Acta
G SomjenT Gilat

Abstract

Phospholipid vesicles, a newly described (bile salt independent) mode of cholesterol transport in human bile, were previously characterized by quasi-elastic light scattering and gel filtration. In the present study the ultrastructure of these vesicles was investigated by electron microscopy using freeze-fracture and negative-staining techniques. Vesicles of varying size were found in all 14 hepatic and 3 gallbladder biles examined. The diameter of the vesicles ranged from 25 to 75 nm by electron microscopy after freeze fracture and from 54 to 94 nm by quasi-elastic light scattering. They had a spherical shape and appeared to be unilamellar. The appearance of the vesicles in fresh hepatic and gallbladder biles as well as in chromatographic fractions was similar. Vesicles were dissolved by the addition of exogenous bile salts. Cholesterol is transported in human bile by both vesicles and micelles. The role of the vesicles may be particularly important in preventing cholesterol precipitation in dilute and supersaturated biles.

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Citations

May 1, 1993·Biochemical Society Transactions·R E HollandD Billington
Jun 10, 1996·Biochimica Et Biophysica Acta·T Gilat, G J Sömjen
Jun 3, 2006·Journal of Lipid Research·Astrid KostersAlbert K Groen
Nov 20, 2008·Journal of Lipid Research·David Q-H WangMartin C Carey
Oct 12, 2013·World Journal of Gastroenterology : WJG·Adnan Adil HismiogullariKhalid Rahman
Jul 7, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yong WangSheng-Dao Zhang
Jul 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L AccatinoC S Koenig
Apr 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H J VerkadeF Kuipers
Dec 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D E CohenM C Carey
Dec 1, 1996·The Journal of Laboratory and Clinical Medicine·W RenooijG P van Berge Henegouwen
Sep 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N H AfdhalG D Offner
Oct 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S SakisakaK Tanikawa
Nov 5, 1997·Microscopy Research and Technique·A KaplunY Talmon
Mar 1, 1992·Journal of Gastroenterology and Hepatology·R KiyosawaF Nakayama
Jan 7, 1993·Biochemical Pharmacology·R E HollandD Billington
Jan 16, 1990·Biochimica Et Biophysica Acta·G J SömjenT Gilat
Oct 15, 1991·Biochimica Et Biophysica Acta·K ChijiiwaF Nakayama
Nov 13, 1991·Biochimica Et Biophysica Acta·A R Beaudoin, G Grondin

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