Cholesteryl ester transfer activity in lipoprotein lipase deficiency and other primary hypertriglyceridemias

Clinica Chimica Acta; International Journal of Clinical Chemistry
A IglesiasM A Lasunción


Cholesteryl ester transfer protein (CETP) activity was measured in d > 1.21 g/ml plasma from hypertriglyceridemic patients and compared with normolipidemic subjects. The assay consisted in measuring the specific transfer of [3H]cholesteryl oleate from a prelabelled, apo E-poor HDL fraction to VLDL after incubation at 37 degrees C in the presence of the d > 1.21 g/ml plasma sample: the lipoproteins were then separated by precipitation with dextran sulfate/Mg2+ solution. Increasing the volume of d > 1.21 g/ml plasma or purified human CETP in the assay produced linear responses in measured activity, whereas, either during incubation at 4 degrees C or in the presence of rat plasma instead of human plasma, the transfer of [3H]cholesteryl oleate to VLDL was not stimulated. Thus, the assay reflects changes in CETP in the sample and appears to be suitable for measuring CETP activity in d > 1.21 g/ml plasma. CETP activity was very similar in the two groups of normolipidemic subjects considered: adolescents (203 +/- 11 nmol esterified cholesterol transferred per 8 h/ml plasma) and adults (215 +/- 5). Patients were grouped into lipoprotein-lipase (LPL)-deficient and non-LPL-deficient according to their enzyme activity in postheparin plasm...Continue Reading


Sep 16, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·J K HuttunenE A Nikkilä
Jun 1, 1992·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·K R MarottiG W Melchior
Apr 1, 1991·European Journal of Clinical Investigation·J D BagdadeP V Subbaiah
Jan 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·A Van TolJ E Groener
Apr 1, 1991·The Journal of Clinical Investigation·J D BagdadeP V Subbaiah
Jan 1, 1990·Annual Review of Nutrition·D W Quig, D B Zilversmit
Jan 1, 1990·The Journal of Clinical Investigation·Y L MarcelR W Milne
May 1, 1986·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·Y S Son, D B Zilversmit
Nov 14, 1986·Biochimica Et Biophysica Acta·D W Quig, D B Zilversmit
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C J FieldingP E Fielding
Jan 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J J AlbersA Steinmetz
Jan 1, 1982·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Y C Ha, P J Barter
Dec 8, 1962·Nature·P B GARLAND, P J RANDLE

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Apr 30, 1996·Clinica Chimica Acta; International Journal of Clinical Chemistry·A IglesiasM A Lasunción
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