PMID: 2498639Jan 23, 1989Paper

Choline transport in Pseudomonas aeruginosa

Molecular and Cellular Biochemistry
M A SalvanoCarlos E Domenech

Abstract

Choline used as the sole carbon or carbon and nitrogen source induces in Pseudomonas aeruginosa an active transport system. The induction of the choline uptake is repressed by succinate independently of the presence of ammonium ion in the culture medium. The repression mediated by succinate was insensitive to cyclic AMP. Substitution for dibutyryl-cyclic AMP was without effect. Choline metabolites that also support the growth of Pseudomonas aeruginosa were poor inducer agents of the choline transport. Kinetic evidence and the employment of choline metabolites as effectors indicated that the choline uptake system of this bacterium is formed by at least two components: one of high affinity (Km = 3 microM) and another of low affinity (Km = 400 microM). Contrary to what occurs in the synaptosome system, the high affinity form for the choline uptake was not dependent on Na+ ions and is not inhibited by hemicholinium-3. Since Pseudomonas aeruginosa can utilize choline as the sole carbon and nitrogen source, the induction of the choline transport with two components in this bacterium may be related to its own strategy to survive and grow in an adverse environment.

Citations

Apr 1, 1993·Comparative Biochemistry and Physiology. Comparative Physiology·R R MillerB W Geer
Apr 2, 2003·Biochimica Et Biophysica Acta·Aran Incharoensakdi, Aphichart Karnchanatat
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E Roberts
Jan 29, 2013·Applied and Environmental Microbiology·Matthew J Wargo
Dec 24, 2008·Infection and Immunity·Matthew J WargoDeborah A Hogan
Dec 12, 2012·Infection and Immunity·Sarah E Clark, Jeffrey N Weiser
Jan 1, 1996·Annual Review of Microbiology·K J Miller, J M Wood
May 22, 1998·FEMS Microbiology Letters·G I LucchesiC E Domenech
Mar 26, 1998·FEMS Microbiology Letters·S T Albelo, C E Domenech

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