Choline uptake into the malaria parasite is energized by the membrane potential

Biochemical and Biophysical Research Communications
Adele M LehaneKiaran Kirk

Abstract

The uptake by the intraerythrocytic malaria parasite of the phospholipid precursor choline was investigated in parasites 'isolated' from their host cells by saponin permeabilization of the erythrocyte membrane. Choline is transported across the parasite plasma membrane then phosphorylated and thereby trapped within the parasite. Choline influx was inhibited competitively by quinine. It increased with increasing extracellular pH, decreased on depolarization of the parasite plasma membrane with a protonophore or by increasing extracellular [K+], and increased in response to hyperpolarization of the membrane by decreasing extracellular [K+] or by addition of the K+ channel blocker Cs+. In ATP-depleted parasites choline was taken up but not phosphorylated. Under these conditions, imposition of an inwardly negative membrane potential using the K+ ionophore valinomycin resulted in the accumulation of choline to an intracellular concentration more than 15-fold higher than the extracellular concentration. Choline influx is therefore an electrogenic process, energized by the parasite plasma membrane potential.

References

Jul 13, 2000·The Journal of Biological Chemistry·T BudimanG Nagel
Mar 29, 2001·Physiological Reviews·K Kirk
Mar 30, 2001·The Journal of Biological Chemistry·K J Saliba, K Kirk
Oct 19, 2001·Molecular and Biochemical Parasitology·L M Alleva, K Kirk
Aug 15, 2002·Biochemical and Biophysical Research Communications·Mari KakehiYasufumi Sawada
Dec 7, 2002·Molecular and Biochemical Parasitology·Rachel Zufferey, Choukri Ben Mamoun
Mar 5, 2003·Molecular and Biochemical Parasitology·Henri J VialJaap J van Hellemond
Nov 25, 2003·The Journal of Biological Chemistry·Richard J W Allen, Kiaran Kirk
Apr 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Gabriella PessiChoukri Ben Mamoun

❮ Previous
Next ❯

Citations

Jun 1, 2005·Trends in Parasitology·Giancarlo A BiaginiPatrick G Bray
Jul 30, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jiannan SongSteven H Zeisel
Dec 6, 2006·Antimicrobial Agents and Chemotherapy·Vinay ChoubeyUday Bandyopadhyay
Sep 3, 2010·BMC Systems Biology·Carola HuthmacherHermann-Georg Holzhütter
Jul 6, 2010·International Journal for Parasitology·Sandrine DéchampsHenri J Vial
Apr 16, 2010·Trends in Parasitology·Henry M StainesSanjeev Krishna
Aug 5, 2008·Molecular and Biochemical Parasitology·Natalie J SpillmanKiaran Kirk
Jun 19, 2010·Drug Development Research·Choukri Ben MamounHenri Vial
Nov 4, 2004·Trends in Parasitology·Katja Becker, Kiaran Kirk
Apr 22, 2006·Biochimica Et Biophysica Acta·Vinay ChoubeyUday Bandyopadhyay
Dec 12, 2013·The Biochemical Journal·Kiaran Kirk, Adele M Lehane
Nov 19, 2014·Bioscience Reports·Rongwei TengKiaran Kirk
Mar 14, 2007·Molecular and Biochemical Parasitology·Tyler N BennettPaul D Roepe
May 19, 2007·Molecular and Biochemical Parasitology·Ping WangJohn E Hyde
Apr 13, 2010·Molecular and Biochemical Parasitology·Terry K Smith, Peter Bütikofer
May 9, 2014·Frontiers in Plant Science·Paul DeanT Martin Embley
Aug 10, 2016·BMC Systems Biology·Anders WallqvistJaques Reifman
Jun 22, 2007·The Journal of Biological Chemistry·Adele M LehaneKevin J Saliba
Jul 24, 2008·Journal of Proteome Research·Jia V LiJürg Utzinger

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.