Cholinergic mediation of alcohol-induced experimental pancreatitis.

Alcoholism, Clinical and Experimental Research
Aurelia LugeaStephen J Pandol

Abstract

The mechanisms initiating pancreatitis in patients with chronic alcohol abuse are poorly understood. Although alcohol feeding has been previously suggested to alter cholinergic pathways, the effects of these cholinergic alterations in promoting pancreatitis have not been characterized. For this study, we determined the role of the cholinergic system in ethanol-induced sensitizing effects on cerulein pancreatitis. Rats were pair-fed control and ethanol-containing Lieber-DeCarli diets for 6 weeks followed by parenteral administration of 4 hourly intraperitoneal injections of the cholecystokinin analog, cerulein at 0.5 μg/kg. This dose of cerulein was selected because it caused pancreatic injury in ethanol-fed but not in control-fed rats. Pancreatitis was preceded by treatment with the muscarinic receptor antagonist atropine or by bilateral subdiaphragmatic vagotomy. Measurement of pancreatic pathology included serum lipase activity, pancreatic trypsin, and caspase-3 activities, and markers of pancreatic necrosis, apoptosis, and autophagy. In addition, we measured the effects of ethanol feeding on pancreatic acetylcholinesterase activity and pancreatic levels of the muscarinic acetylcholine receptors m1 and m3. Finally, we examine...Continue Reading

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Citations

Mar 5, 2011·The Journal of Biological Chemistry·Abrahim I OrabiSohail Z Husain
Feb 11, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kamaldeen A MuiliSohail Z Husain
Nov 30, 2010·Gastroenterology·Aurelia LugeaStephen J Pandol
Feb 3, 2011·Alcoholism, Clinical and Experimental Research·Stephen J PandolIlya Gukovsky
Jul 16, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xianbao ZhanBaoan Ji
Jul 19, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yannan LiuStephen J Pandol
Oct 21, 2020·Cellular and Molecular Gastroenterology and Hepatology·Michelle M CooleyGuy E Groblewski
Apr 22, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Melinda Wang, Fred Sanford Gorelick

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