Abstract
Cholinergic regulation of baker's yeast cell phagocytosis in rat cultured astrocytes was studied. Phagocytic activity was reduced by 1 x 10(-5) M of atropine or pirenzepine, but not by AF-DX116 or 4-DAMP. In addition, carbachol stimulated phagocytosis in a dose-dependent manner. Furthermore, only 1 x 10(-5)M of atropine, pirenzepine and 4-DAMP significantly reduced enhanced activity induced by 1 x 10(-7)M carbachol. It was also observed that L-NMMA, staurosporine, or U-73122, reduced phagocytosis activity while TFP failed to do so. Nitrite levels in astrocyte supernatants increased after baker's yeast cells were incorporated to astrocyte cultures, correlating with enhanced phagocytosis induced by carbachol stimulation, and were reduced by 1 x 10(-5) M of atropine, pirenzepine or aminopiridine, but not by AF-DX116 or 4-DAMP. Enhanced NO production triggered by astrocyte phagocytosis may have pathological consequences.
References
Oct 1, 1991·Journal of Neuroscience Methods·R F IaconoE F Lascano
Nov 7, 1988·FEBS Letters·T AnderssonO Stendahl
Jan 1, 1985·Acta Neuropathologica·M I Berría, E F Lascano
Jan 1, 1984·Progress in Neuro-psychopharmacology & Biological Psychiatry·L HertzA Schousboe
May 1, 1993·Glia·E A Van Der ZeeP G Luiten
Feb 22, 1996·European Journal of Pharmacology·M GuizzettiL G Costa
Oct 1, 1995·Neurochemistry International·C L AtanassovN Seiler
Jun 1, 1997·Glia·I Bechmann, R Nitsch
Dec 14, 1999·Glia·S Murphy
Jan 15, 2000·Journal of Neuroimmunology·M E Smith, M T Hoerner
Sep 26, 2000·Cytometry·M C CatlinL G Costa
Jul 21, 2001·The Biochemical Journal·W K AldertonR G Knowles
Aug 23, 2001·Neuroscience·A Cedazo-MínguezT G Ohm
Sep 13, 2002·Journal of Neuroimmunology·Tim MagnusRalf Gold
Jul 5, 2003·Neuropharmacology·Leonor Sterin-BordaEnri Borda