Cholinergic modulation of dopaminergic neurons in the mouse olfactory bulb

Chemical Senses
Angela Pignatelli, Ottorino Belluzzi

Abstract

Considerable evidence exists for an extrinsic cholinergic influence in the maturation and function of the main olfactory bulb. In this study, we addressed the muscarinic modulation of dopaminergic neurons in this structure. We used different patch-clamp techniques to characterize the diverse roles of muscarinic agonists on identified dopaminergic neurons in a transgenic animal model expressing a reporter protein (green fluorescent protein) under the tyrosine hydroxylase promoter. Bath application of acetylcholine (1 mM) in slices and in enzymatically dissociated cells reduced the spontaneous firing of dopaminergic neurons recorded in cell-attached mode. In whole-cell configuration no effect of the agonist was observed, unless using the perforated patch technique, thus suggesting the involvement of a diffusible second messenger. The effect was mediated by metabotropic receptors as it was blocked by atropine and mimicked by the m2 agonist oxotremorine (10 muM). The reduction of periglomerular cell firing by muscarinic activation results from a membrane-potential hyperpolarization caused by activation of a potassium conductance. This modulation of dopaminergic interneurons may be important in the processing of sensory information ...Continue Reading

References

Feb 1, 1990·Brain Research Bulletin·N RavelG Chouvet
Nov 1, 1983·Neuroscience·N Halász, G M Shepherd
Jul 15, 1983·Experientia·N G LindquistH Samaan
Oct 8, 1993·The Journal of Comparative Neurology·H Le Jeune, F Jourdan
Jan 1, 1997·Life Sciences·D A BrownA A Selyanko
Sep 30, 1998·The European Journal of Neuroscience·M Puopolo, O Belluzzi
Aug 13, 1999·Journal of Neurophysiology·A Y HsiaP M Lledo
Jan 29, 2000·Brain Research·D A Berkowicz, P Q Trombley
Nov 9, 2000·The European Journal of Neuroscience·C CrespoF J Martínez-Guijarro
May 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·K SawamotoH Okano
Dec 4, 2001·Journal of Neurophysiology·M EnnisM T Shipley
Jul 19, 2002·Journal of Neurochemistry·Natsuki MatsushitaKazuto Kobayashi
Mar 4, 2003·Journal of Neurophysiology·Nestor G DavilaPaul Q Trombley
Feb 26, 2005·The Journal of Physiology·Angela PignatelliOttorino Belluzzi
Dec 2, 2005·Journal of Neurophysiology·Ambarish S GhatpandeSukumar Vijayaraghavan
Sep 27, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Naoko KanekoKazunobu Sawamoto
Dec 13, 2006·The European Journal of Neuroscience·Nathalie MandaironChristiane Linster

❮ Previous
Next ❯

Citations

Oct 29, 2010·Learning & Memory·Max L Fletcher, Wei R Chen
Sep 24, 2010·Journal of Neurophysiology·Richard S Smith, Ricardo C Araneda
Feb 28, 2013·Journal of Neural Transmission·Guangwei LiuShun Yu
Dec 24, 2011·Neurobiology of Disease·Richard L Doty
Oct 8, 2008·Neuroscience Letters·Nicolaas I BohnenRobert Y Moore
Aug 19, 2009·Annals of the New York Academy of Sciences·Alan Gelperin, Ambarish Ghatpande
Apr 9, 2015·Frontiers in Neuroanatomy·Teresa LiberiaCarlos Crespo
Nov 13, 2012·Cell Reports·Christian J NiedworokMartin K Schwarz
May 12, 2016·Scientific Reports·Mounir BendahmaneMax L Fletcher
Nov 27, 2007·Progress in Brain Research·Noriko U Takemura, Nobumasa Kato
Aug 5, 2016·The Journal of Comparative Neurology·Masakazu HamamotoKazunori Toida
Aug 6, 2019·Frontiers in Behavioral Neuroscience·Jordan M RossMax L Fletcher
Jul 3, 2020·Scientific Reports·Erik BöhmMarkus Rothermel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.