Cholinergic regulation along the pulmonary arterial tree of the South American rattlesnake: vascular reactivity, muscarinic receptors, and vagal innervation

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Renato FilogonioTobias Wang

Abstract

Vascular tone in the reptilian pulmonary vasculature is primarily under cholinergic, muscarinic control, exerted via the vagus nerve. This control has been ascribed to a sphincter located at the arterial outflow, but we speculated whether the vascular control in the pulmonary artery is more widespread, such that responses to acetylcholine and electrical stimulation, as well as the expression of muscarinic receptors is prevalent along its length. Working on the South American rattlesnake (Crotalus durissus), we studied four different portions of the pulmonary artery (truncus, proximal, distal and branches). Acetylcholine elicited robust vasoconstriction in the proximal, distal and branch portions, but the truncus vasodilated. Electrical field stimulation (EFS) caused contractions in all segments, an effect partially blocked by atropine. We identified all five subtypes of muscarinic receptors (M1-M5). The expression of the M1 receptor was largest in the distal end and branches of the pulmonary artery, while expression of the muscarinic M3 receptor was markedly larger in the truncus of the pulmonary artery. Application of the neural tracer DiI revealed widespread innervation along the whole pulmonary artery and retrograde transpor...Continue Reading

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Citations

Feb 26, 2021·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Renato FilogonioDane A Crossley
Jul 6, 2021·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Renato FilogonioDane A Crossley

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Methods Mentioned

BETA
light microscopy
PCR
electrophoresis
RSA

Software Mentioned

ImageJ
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