Chondrocyte deformation induces mitochondrial distortion and heterogeneous intracellular strain fields

Biomechanics and Modeling in Mechanobiology
M M KnightD L Bader

Abstract

Chondrocyte mechanotransduction is poorly understood but may involve cell deformation and associated distortion of intracellular structures and organelles. This study quantifies the intracellular displacement and strain fields associated with chondrocyte deformation and in particular the distortion of the mitochondria network, which may have a role in mechanotransduction. Isolated articular chondrocytes were compressed in agarose constructs and simultaneously visualised using confocal microscopy. An optimised digital image correlation technique was developed to calculate the local intracellular displacement and strain fields using confocal images of fluorescently labelled mitochondria. The mitochondria formed a dynamic fibrous network or reticulum, which co-localised with microtubules and vimentin intermediate filaments. Cell deformation induced distortion of the mitochondria, which collapsed in the axis of compression with a resulting loss of volume. Compression generated heterogeneous intracellular strain fields indicating mechanical heterogeneity within the cytoplasm. The study provides evidence supporting the potential involvement of mitochondrial deformation in chondrocyte mechanotransduction, possibly involving strain-med...Continue Reading

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Citations

Nov 4, 2006·Biomechanics and Modeling in Mechanobiology·Gerard A Ateshian
Aug 30, 2008·Medical & Biological Engineering & Computing·D L Bader, M M Knight
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