Chondrocyte differentiation of human endometrial gland-derived MSCs in layered cell sheets

TheScientificWorldJournal
Waki SekineTeruo Okano

Abstract

Recently, regenerative medicine using engineered three-dimensional (3D) tissues has been focused. In the fields of cell therapy and regenerative medicine, mesenchymal stem cells (MSCs) are attractive autologous cell sources. While, in bioengineered tissues, a 3D environment may affect the differentiation of the stem cells, little is known regarding the effect of 3D environment on cellular differentiation. In this study, MSC differentiation in in vitro 3D tissue models was assessed by human endometrial gland-derived MSCs (hEMSCs) and cell sheet technology. hEMSC sheets were layered into cell-dense 3D tissues and were cultured on porous membranes. The tissue sections revealed that chondrocyte-like cells were found within the multilayered cell sheets even at 24 h after layering. Immunostainings of chondrospecific markers were positive within those cell sheet constructs. In addition, sulfated glycosaminoglycan accumulation within the tissues increased in proportion to the numbers of layered cell sheets. The findings suggested that a high cell density and hypoxic environment in 3D tissues by layering cell sheets might accelerate a rapid differentiation of hEMSCs into chondrocytes without the help of chondro-differentiation reagents....Continue Reading

References

Sep 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A I Caplan
Oct 6, 1994·The New England Journal of Medicine·M BrittbergL Peterson
Apr 1, 1994·The Journal of Bone and Joint Surgery. American Volume·S WakitaniV M Goldberg
Apr 2, 1999·Science·M F PittengerD R Marshak
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·D C ColterD J Prockop
Jun 5, 2002·The Journal of Bone and Joint Surgery. British Volume·M OchiJ Iwasa
Aug 22, 2003·Nature
Aug 22, 2003·Nature·Alison Abbott
Sep 17, 2004·The New England Journal of Medicine·Kohji NishidaYasuo Tano
Jan 28, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tatsuya ShimizuTeruo Okano
Jul 28, 2006·Nature·Jamil El-AliKlavs F Jensen
Sep 5, 2006·Biochemical and Biophysical Research Communications·Nagatoshi KaneshiroJoji Mochida
Dec 14, 2006·In Vitro Cellular & Developmental Biology. Animal·Keiran S M SmalleyMeenhard Herlyn
Jun 12, 2008·Medical & Biological Engineering & Computing·Masato SatoJoji Mochida
Nov 21, 2008·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Junji IwasaMitsuo Ochi
Mar 10, 2009·BMC Biotechnology·Genya MitaniJoji Mochida
Aug 14, 2009·Expert Review of Cardiovascular Therapy·Juan C Chachques
Apr 14, 2010·Annals of Biomedical Engineering·Yoshikazu KumashiroTeruo Okano
May 12, 2010·Journal of Cellular Physiology·Tomiya MatsumotoShigeyuki Wakitani
Sep 29, 2011·Journal of Tissue Engineering and Regenerative Medicine·H FazekasovaGiovanna Lombardi
Dec 27, 2011·Frontiers of Medicine·Shihua WangRobert Chunhua Zhao
Jun 29, 2012·Circulation Journal : Official Journal of the Japanese Circulation Society·Sung Hyun ChoiSang Hong Baek
Aug 18, 2012·Tissue Engineering. Part B, Reviews·Sarah E BulmanFrank Barry
Dec 1, 2012·Stem Cells Translational Medicine·Yuji HaraguchiTeruo Okano

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Citations

Nov 8, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Akiyuki HasegawaTatsuya Shimizu
Jun 9, 2016·Journal of Functional Biomaterials·Azizah Intan PangestyMitsugu Todo
Mar 7, 2017·Current Stem Cell Reports·Doris A TaylorLuiz C Sampaio
Mar 31, 2021·Npj Regenerative Medicine·Andrea De PieriDimitrios I Zeugolis

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