Chondrocyte protein with a poly-proline region (CHPPR) is a novel mitochondrial protein and promotes mitochondrial fission

Journal of Cellular Physiology
L TonachiniP Castagnola

Abstract

We have recently identified a chondrocyte protein with a poly-proline region, referred to as CHPPR, and showed that this protein is expressed intracellularly in chick embryo chondrocytes. Conventional fluorescence and confocal localization of CHPPR shows that CHPPR is sorted to mitochondria. Furthermore, immunoelectron microscopy of CHPPR transfected cells demonstrates that this protein is mostly associated with the mitochondrial inner membranes. Careful analysis of CHPPR expressing cells reveals, instead of the regular mitochondrial tubular network, the presence of a number of small spheroid mitochondria. Here we show that the domain responsible for network-spheroid transition spans amino acid residues 182-309 including the poly-proline region. Functional analyses of mitochondrial activity rule out the possibility of mitochondrial damage in CHPPR transfected cells. Since cartilage expresses high levels of CHPPR mRNA when compared to other tissues and because CHPPR is associated with late stages of chondrocyte differentiation, we have investigated mitochondrial morphology in hypertrophic chondrocytes by MitoTracker Orange labeling. Confocal microscopy shows that these cells have spheroid mitochondria. Our data demonstrate that ...Continue Reading

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Citations

Jun 9, 2009·PloS One·Jingjing GuEmmeline W Hill
Sep 4, 2009·International Journal of Biological Sciences·Zhihua JiangMichael D Macneil
Aug 22, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Massimiliano MonticonePatrizio Castagnola
Sep 28, 2005·The Journal of Investigative Dermatology·Susan TamijiRenata Polakowska
Jun 23, 2010·Journal of Cellular Physiology·Massimiliano MonticonePatrizio Castagnola
May 29, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Katia CortesePatrizio Castagnola
Nov 8, 2011·Neurobiology of Aging·Philip L De JagerDenis A Evans
Jun 28, 2016·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Hengjian XuQingxi J Shen
Jul 23, 2020·Nature Metabolism·Susanne SeitzAnja Zeigerer
Jan 29, 2021·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Liuyan WuZhaoxin Tang
Nov 12, 2020·Cancer Management and Research·Wenjie LuYang Wang
Sep 21, 2021·The EMBO Journal·Sergio Guerrero-CastilloSusanne Arnold

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