Chondrogenesis of human bone marrow mesenchymal stromal cells in highly porous alginate-foams supplemented with chondroitin sulfate

Materials Science & Engineering. C, Materials for Biological Applications
Zhao HuangGundula Schulze-Tanzil

Abstract

To overcome the limited intrinsic cartilage repair, autologous chondrocyte or bone-marrow-derived mesenchymal stromal cell (BM-MSC) was implanted into cartilage defects. For this purpose suitable biocompatible scaffolds are needed to provide cell retention, chondrogenesis and initial mechanical stability. The present study should indicate whether a recently developed highly porous alginate (Alg) foam scaffold supplemented with chondroitin sulfate (CS) allows the attachment, survival and chondrogenesis of BM-MSCs and articular chondrocytes. The foams were prepared using a freeze-drying method; some of them were supplemented with CS and subsequently characterized for porosity, biodegradation and mechanical profile. BM-MSCs were cultured for 1-2 weeks on the scaffold either under chondrogenic or maintenance conditions. Cell vitality assays, histology, glycosaminoglycan (sGAG) assay, and type II and I collagen immunolabelings were performed to monitor cell growth and extracellular matrix (ECM) synthesis in the scaffolds. Scaffolds had a high porosity ~93-95% with a mean pore sizes of 237±48 μm (Alg) and 197±61 μm (Alg/CS). Incorporation of CS increased mechanical strength of the foams providing gradually CS release over 7 days. Mos...Continue Reading

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Citations

Mar 15, 2016·Osteoarthritis and Cartilage·M K MamidiR Bhonde
Jul 1, 2016·Journal of Biomaterials Applications·Vineet GuptaMichael S Detamore
May 3, 2019·Stem Cell Research & Therapy·Nádia de Cássia NoronhaKelen C R Malmegrim
Mar 11, 2017·Journal of Orthopaedic Surgery and Research·Andy GoldbergRazi Zaidi
Feb 16, 2021·Frontiers in Bioengineering and Biotechnology·Sarah DavisTosca Roncada
Oct 6, 2018·Carbohydrate Polymers·Ovidio CatanzanoGiovanna Gomez d'Ayala
Jan 20, 2021·ACS Biomaterials Science & Engineering·Yasaman AlinejadSophie Lerouge

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