Chondrogenesis of synovium-derived mesenchymal stem cells in gene-transferred co-culture system

Biomaterials
Rohan R VarshneyDong-An Wang

Abstract

A co-culture strategy has been developed in this study wherein rabbit synovial mesenchymal stem cells (SMSCs) are co-cultured with growth factor (GF) transfected articular chondrocytes. Toward this end, both SMSCs and early passage rabbit articular chondrocytes that had been adenovirally transduced with transforming growth factor-beta 3 (TGF-beta3) gene were separately encapsulated in alginate beads and co-cultured in the same pool of chondrogenic medium. The chondrocytes act as transfected companion cells (TCCs) providing GF supply to induce chondrogenic differentiation of SMSCs that play the role of therapeutic progenitor cells (TPCs). Against the same TCC based TGF-beta3 release profile, the co-culture was started at different time points (Day 0, Day 10 and Day 20) but made to last for identical periods of exposure (30 days) so that the exposure conditions could be optimized in terms of initiation and duration. Transfection of TCCs prevents the stem cell based TPCs from undergoing the invasive procedure. It also prevents unpredictable complications in the TPCs caused by long-term constitutive over-expression of a GF. The adenovirally transfected TCCs exhibit a transient GF expression which results in a timely termination of ...Continue Reading

Citations

Jul 13, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Avital MendelsonJeremy J Mao
Jul 6, 2014·BioMed Research International·Shwu Jen ChangShan-Wei Yang
Oct 19, 2011·Journal of Biomedicine & Biotechnology·Seogjin Seo, Kun Na
Feb 21, 2014·Tissue Engineering. Part B, Reviews·Gun-Il Im
Sep 2, 2014·Advanced Drug Delivery Reviews·Martin J StoddartMauro Alini
Nov 13, 2015·Tissue Engineering. Part B, Reviews·Gun-Il Im
Apr 17, 2013·Biotechnology and Applied Biochemistry·Aruna SanthagunamJoaquim M S Cabral
Nov 21, 2012·Journal of Tissue Engineering and Regenerative Medicine·Feng ZhangDong-An Wang
Aug 12, 2015·Chemical Reviews·Yulin LiHelena Tomás
May 13, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Narendra K Singh, Doo Sung Lee
Jul 19, 2014·Biochemical and Biophysical Research Communications·Yong Il KimYong-Gon Koh
May 27, 2017·Journal of Cellular Physiology·Yu ZhangQuanyi Guo
Oct 9, 2018·Expert Opinion on Biological Therapy·Jianyu ZouYongchang Yao
Mar 11, 2017·Journal of Orthopaedic Surgery and Research·Andy GoldbergRazi Zaidi
Feb 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Puxiu WangXing Tang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.