Chondrogenic differentiation of human mesenchymal stem cells in micro-masses is impaired by high doses of the chemokine CXCL7

Journal of Tissue Engineering and Regenerative Medicine
G KalwitzChristian Kaps

Abstract

Chemokines have been shown to recruit human mesenchymal stem cells (MSCs) and are suggested to be promising candidates for in situ tissue engineering. The aim of our study was to analyse the effect of CXCL7, a chemokine that has the capacity to recruit MSCs, on the chondrogenic differentiation of MSCs. Bone marrow-derived MSCs were cultured in high-density micro-masses under serum-free conditions and were co-stimulated with 0-100 nM CXCL7 in the presence of 10 ng/ml transforming growth factor-β3 (TGFβ3). Micro-masses stimulated without growth factors and chemokines served as controls. Histological staining of proteoglycan, immunostaining of type II collagen, staining of mineralized matrix according to von Kossa as well as real-time gene expression analysis of typical chondrogenic and osteogenic marker genes showed that the TGFβ3-mediated chondrogenic development of MSCs was not impaired by 0-50 nM CXCL7. Micro-masses stimulated with TGFβ3 and CXCL7 developed chondrogenic cells and formed a cartilaginous matrix rich in proteoglycans, accompanied by the induction of typical chondrogenic marker genes, such as cartilage oligomeric matrix protein, aggrecan, type IIα1 collagen and by regulation of matrix metalloproteinases and their ...Continue Reading

References

Feb 11, 1998·Experimental Cell Research·B JohnstoneJ U Yoo
Apr 2, 1999·Science·M F PittengerD R Marshak
Aug 8, 2001·Journal of Internal Medicine·M Baggiolini
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ichiro SekiyaDarwin J Prockop
Oct 25, 2002·Journal of Cellular Biochemistry·Jörg FiedlerRolf E Brenner
Nov 19, 2002·Die Naturwissenschaften·Jochen RingeMichael Sittinger
Oct 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Denise MerzRobert Terkeltaub
Dec 23, 2003·Differentiation; Research in Biological Diversity·Bernhard SchmittChristian Kaps
Mar 11, 2004·The International Journal of Biochemistry & Cell Biology·Frank P Barry, J Mary Murphy
Aug 4, 2004·Cellular and Molecular Life Sciences : CMLS·W X FuW F Chen
Oct 14, 2004·Clinical Orthopaedics and Related Research·Rosa Maria BorzìAndrea Facchini
May 24, 2006·Biochemical and Biophysical Research Communications·Jörg FiedlerRolf E Brenner
Jan 18, 2007·Journal of Cellular Physiology·Antonio GiordanoIgnazio R Marino
Mar 30, 2007·Arthritis Research & Therapy·Farida DjouadChristian Jorgensen
Jun 7, 2007·Molecular Pharmacology·Yung-Cheng ChiuChih-Hsin Tang
Sep 1, 2007·Current Opinion in Rheumatology·Wiltrud Richter
Jan 22, 2008·Arthritis Research & Therapy·Kristin AndreasMichael Sittinger
Apr 3, 2008·Journal of Tissue Engineering and Regenerative Medicine·J RingeM Sittinger
May 9, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yasunori Mishima, Martin Lotz
Aug 19, 2008·The International Journal of Biochemistry & Cell Biology·Gregor KalwitzChristian Kaps
Nov 22, 2008·The Journal of Pathology·A I Caplan
Mar 14, 2009·Journal of Tissue Engineering and Regenerative Medicine·Aditee Kurane, Naren Vyavahare

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Citations

Nov 20, 2014·International Journal of Molecular Sciences·Masahiro KondoYoshiya Tanaka
Dec 14, 2016·Oncology Reports·Qian GuoLiang Chang

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