Chondrogenic differentiation of mouse embryonic stem cells promoted by mature chondrocytes

Science in China. Series C, Life Sciences
Feng XieYiLin Cao

Abstract

In order to direct embryonic stem (ES) cells to differentiate into chondrocytes, a chondrogenic environment provided by mature chondrocytes was investigated. Flk-1 positive cells sorted from pre-differentiated mouse ES cells were mixed with adult porcine articular chondrocytes, seeded on biodegradable scaffolds, and then implanted subcutaneously into nude mice. The cell-scaffold complexes formed cartilage tissues after 4 weeks, which was demonstrated by histology and anti-type II collagen antibody staining. Positive staining of mouse Major Histocompatibility Complex class I molecules confirmed that part of the chondrocytes were derived from mouse ES cells. The current study established a new approach for directing ES cell differentiation.

References

Apr 8, 2003·Journal of Cell Science·Naoki NakayamaChun-Ya E Han
Dec 23, 2003·Differentiation; Research in Biological Diversity·Yaping SuiJaspal Singh Khillan
Mar 1, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C W ChenW F Lai

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Citations

Apr 16, 2011·Pharmaceutical Research·Nathaniel S HwangDaniel G Anderson
Jun 30, 2010·Molecular Pharmaceutics·Bradley A BordenSung Wan Kim
Aug 21, 2013·Tissue Engineering. Part B, Reviews·Aixin ChengSusan J Kimber

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