Chondrogenic differentiation potential of adult and fetal equine cell types

Veterinary Surgery : VS
Emma N AdamJames N MacLeod

Abstract

To determine the chondrogenic potential of cells derived from interzone tissue, the normal progenitor of articular cartilage during fetal development, compared to that of adult bone marrow-derived and adipose-derived mesenchymal cell isolates. The objective of this study was to compare the chondrogenic potential of fetal musculoskeletal progenitor cells to adult cell types, which are currently used therapeutically to facilitate joint cartilage repair in equine clinical practice. The hypothesis tested was that cells derived from interzone tissue have a chondrogenic potential that exceeds that of adult bone marrow-derived and adipose-derived mesenchymal cell isolates. In vitro study. Six young adult horses (15-17 months of age) and 6 equine fetuses aged 45-46 days of gestation. Three-dimensional pellet cultures were established under chondrogenic conditions with fresh, primary cells isolated from adult (articular cartilage, bone marrow, adipose, dermis) and fetal (interzone, skeletal anlagen cartilage, dermis) tissues. Cellular morphology, pellet architecture, and proteoglycan synthesis were assessed in the pellet cultures. Steady state levels of ACAN (aggrecan core protein), COL2A1 (collagen type II), and COL1A1 (collagen type I...Continue Reading

References

Jul 17, 1975·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·I A Silver
Jan 1, 1990·Annals of the New York Academy of Sciences·M PacificiS L Adams
Oct 6, 1994·The New England Journal of Medicine·M BrittbergL Peterson
Jan 26, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M C StewartJ N Macleod
Oct 18, 2001·Clinical Orthopaedics and Related Research·J R SteadmanJ J Rodrigo
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 6, 2003·Neuroscience Letters·Christian RamakersAntoon F M Moorman
Jan 24, 2006·The Veterinary Journal·Laurie R Goodrich, Alan J Nixon
Jul 13, 2006·Annals of the New York Academy of Sciences·Maurizio PacificiMasahiro Iwamoto
Sep 14, 2006·Tissue Engineering·Shawn Patrick GroganPierre Mainil-Varlet
May 23, 2007·BioTechniques·Melina V Jones, Peter A Calabresi
Aug 19, 2007·Differentiation; Research in Biological Diversity·Samoa GiovanniniDobrila Nesic
Oct 26, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·John D KisidayDavid D Frisbie
Dec 18, 2007·Annals of the New York Academy of Sciences·Eiki KoyamaMaurizio Pacifici
Jun 23, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·David D FrisbieC Wayne McIlwraith
Jan 26, 2010·Pathology, Research and Practice·Ivyna Bong Pau NiSharifah Noor Akmal Syed Hussain
Dec 1, 2010·Osteoarthritis and Cartilage·R S CosdenJ N MacLeod
Feb 15, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Alan J NixonGloria L Matthews
Nov 8, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jan Philipp KrügerChristian Kaps
Jul 18, 2012·Osteoarthritis and Cartilage·R S Cosden-DeckerJ N MacLeod
Jan 16, 2013·Cytotherapy·Mahmood Saba ChoudheryDavid T Harris
Apr 24, 2013·Bone & Joint Research·C W McIlwraithC E Kawcak
Sep 21, 2013·Methods in Molecular Biology·Andrea Streit, Claudio D Stern
Aug 31, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Rebekah S DeckerMaurizio Pacifici
Jun 17, 2015·Molecular and Cellular Neurosciences·Lena BraunschweigAlexander Storch
Oct 23, 2015·Mediators of Inflammation·Hemant Kumar, Dong-Kug Choi
Nov 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Angelo S Mao, David J Mooney
Jan 8, 2016·The Journal of Bone and Joint Surgery. American Volume·Laurie R GoodrichConstance R Chu
Mar 5, 2016·Nature Communications·Allison J CoteRobert L Mauck

❮ Previous
Next ❯

Citations

Aug 16, 2019·PloS One·Eugene W HindererHunter N B Moseley
Jul 13, 2021·World Journal of Stem Cells·Joice FülberRaquel Y A Baccarin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

© 2022 Meta ULC. All rights reserved