Chondrogenic properties of collagen type XI, a component of cartilage extracellular matrix

Biomaterials
Ang LiGordana Vunjak-Novakovic

Abstract

Cartilage extracellular matrix (ECM) has been used for promoting tissue engineering. However, the exact effects of ECM on chondrogenesis and the acting mechanisms are not well understood. In this study, we investigated the chondrogenic effects of cartilage ECM on human mesenchymal stem cells (MSCs) and identified the contributing molecular components. To this end, a preparation of articular cartilage ECM was supplemented to pellets of chondrogenically differentiating MSCs, pellets of human chondrocytes, and bovine articular cartilage explants to evaluate the effects on cell proliferation and the production of cartilaginous matrix. Selective enzymatic digestion and screening of ECM components were conducted to identify matrix molecules with chondrogenic properties. Cartilage ECM promoted MSC proliferation, production of cartilaginous matrix, and maturity of chondrogenic differentiation, and inhibited the hypertrophic differentiation of MSC-derived chondrocytes. Selective digestion of ECM components revealed a contributory role of collagens in promoting chondrogenesis. The screening of various collagen subtypes revealed strong chondrogenic effect of collagen type XI. Finally, collagen XI was found to promote production and inhibi...Continue Reading

Citations

Jun 21, 2019·Annals of Biomedical Engineering·Salma MahzoonMichael S Detamore
May 20, 2020·Frontiers in Bioengineering and Biotechnology·Weiping LinGang Li
Apr 25, 2020·Communications Biology·Llorenç Solé-BoldoFrank Lyko
Mar 11, 2020·Polymers·Andrea Catalina Villamil BallesterosYuli Alexandra Neira Bejarano
Jan 18, 2020·Scientific Reports·Yoshihiro IshikawaHans Peter Bächinger
Sep 15, 2020·Journal of Tissue Engineering·Hanxiang LeJianxun Ding
May 18, 2020·International Journal of Molecular Sciences·Arnaud BianchiElmira Arab-Tehrany
Dec 10, 2020·Journal of Tissue Engineering·Zhonghan WangXiaoyu Yang
May 22, 2021·Frontiers in Bioengineering and Biotechnology·Fu WeiQuanyi Guo

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