Abstract
Cartilage extracellular matrix (ECM) has been used for promoting tissue engineering. However, the exact effects of ECM on chondrogenesis and the acting mechanisms are not well understood. In this study, we investigated the chondrogenic effects of cartilage ECM on human mesenchymal stem cells (MSCs) and identified the contributing molecular components. To this end, a preparation of articular cartilage ECM was supplemented to pellets of chondrogenically differentiating MSCs, pellets of human chondrocytes, and bovine articular cartilage explants to evaluate the effects on cell proliferation and the production of cartilaginous matrix. Selective enzymatic digestion and screening of ECM components were conducted to identify matrix molecules with chondrogenic properties. Cartilage ECM promoted MSC proliferation, production of cartilaginous matrix, and maturity of chondrogenic differentiation, and inhibited the hypertrophic differentiation of MSC-derived chondrocytes. Selective digestion of ECM components revealed a contributory role of collagens in promoting chondrogenesis. The screening of various collagen subtypes revealed strong chondrogenic effect of collagen type XI. Finally, collagen XI was found to promote production and inhibi...Continue Reading
Citations
Jun 20, 2019·Polymers·Cai WangYiping Wang
Jun 21, 2019·Annals of Biomedical Engineering·Salma MahzoonMichael S Detamore
May 20, 2020·Frontiers in Bioengineering and Biotechnology·Weiping LinGang Li
Sep 11, 2020·International Journal of Genomics·Hang SuLi Liu
Apr 18, 2019·Journal of Molecular Histology·Susan M Smith, James Melrose
Apr 25, 2020·Communications Biology·Llorenç Solé-BoldoFrank Lyko
Mar 11, 2020·Polymers·Andrea Catalina Villamil BallesterosYuli Alexandra Neira Bejarano
Jan 18, 2020·Scientific Reports·Yoshihiro IshikawaHans Peter Bächinger
Sep 15, 2020·Journal of Tissue Engineering·Hanxiang LeJianxun Ding
May 18, 2020·International Journal of Molecular Sciences·Arnaud BianchiElmira Arab-Tehrany
Apr 23, 2019·Autophagy·Hongxu YangMeiqing Wang
Dec 10, 2020·Journal of Tissue Engineering·Zhonghan WangXiaoyu Yang
Apr 4, 2021·Acta Biomaterialia·Guangzhao TianShuyun Liu
Apr 8, 2021·Stem Cells International·Kangkang ZhaShuyun Liu
May 22, 2021·Frontiers in Bioengineering and Biotechnology·Fu WeiQuanyi Guo
Jun 19, 2021·Nature Communications·Cheng-Cheng DengBin Yang
Jun 18, 2021·Bioactive Materials·Wenying Wei, Honglian Dai
Jul 29, 2021·Bone & Joint Research·Jie YangZandong Zhao
Oct 1, 2021·Frontiers in Cell and Developmental Biology·Yu JiangGuoxia Yu