Chondroitin / dermatan sulfate modification enzymes in zebrafish development

PloS One
Judith HabicherJohan Ledin

Abstract

Chondroitin/dermatan sulfate (CS/DS) proteoglycans consist of unbranched sulfated polysaccharide chains of repeating GalNAc-GlcA/IdoA disaccharide units, attached to serine residues on specific proteins. The CS/DS proteoglycans are abundant in the extracellular matrix where they have essential functions in tissue development and homeostasis. In this report a phylogenetic analysis of vertebrate genes coding for the enzymes that modify CS/DS is presented. We identify single orthologous genes in the zebrafish genome for the sulfotransferases chst7, chst11, chst13, chst14, chst15 and ust and the epimerase dse. In contrast, two copies were found for mammalian sulfotransferases CHST3 and CHST12 and the epimerase DSEL, named chst3a and chst3b, chst12a and chst12b, dsela and dselb, respectively. Expression of CS/DS modification enzymes is spatially and temporally regulated with a large variation between different genes. We found that CS/DS 4-O-sulfotransferases and 6-O-sulfotransferases as well as CS/DS epimerases show a strong and partly overlapping expression, whereas the expression is restricted for enzymes with ability to synthesize di-sulfated disaccharides. A structural analysis further showed that CS/DS sulfation increases durin...Continue Reading

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Citations

Oct 20, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sudhanshu SahuMelitta Schachner
Jan 30, 2018·Frontiers in Molecular Neuroscience·Adam LabadorfRichard H Myers
Jul 17, 2020·The European Journal of Neuroscience·Ismary Blanco, Katherine Conant

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Methods Mentioned

BETA
targeted mutations
PCR
ion exchange chromatography

Software Mentioned

Adobe Illustrator
dsela
CONSENSE
Phylip
SEQBOOT
Adobe Photoshop
PROTDIST
Ensembl

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