PMID: 6413515Oct 1, 1983Paper

Chondroitin sulfate at the plasma membranes of cultured fibroblasts

The Journal of Cell Biology
K HedmanA Vaheri

Abstract

We have previously shown that in confluent human fibroblast cultures chondroitin sulfate proteoglycan is a component of the fibronectin-containing pericellular matrix fibers. In the present work the distribution of chondroitin sulfate was studied in subconfluent cell cultures using antibodies that bind to a chemically defined carbohydrate fragment of chondroitinase ABC-modified chondroitin sulfate proteoglycan. Using immunofluorescence microscopy, we observed, in addition to the fibrillar matrix staining, chondroitin sulfate diffusely distributed at the cell surface. In indirect immunoferritin electron microscopy this staining corresponded to patchy binding of ferritin close (24 nm) to the outer aspect of the plasma membrane. The patchy organization appeared uniform in all cell surfaces. The cell surface chondroitin sulfate could not be removed from the plasma membrane by agents that dissociate electrostatic interactions. These data show that in fibroblasts chondroitin sulfate is a component of the outer aspect of the plasma membrane, and raise the possibility of an integral plasma membrane chondroitin sulfate proteoglycan.

References

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Citations

Jan 1, 1991·The International Journal of Biochemistry·F BertrandJ Picard
May 1, 1986·Journal of the Neurological Sciences·A BertolottoG Tarone
Jun 1, 1987·The Journal of Cell Biology·G SchmidtH Kresse
Mar 1, 1989·The Journal of Cell Biology·G DavidH Van den Berghe
Nov 1, 1986·The Journal of Experimental Medicine·K S GiacolettoB D Schwartz
Mar 1, 1987·Archives of Biochemistry and Biophysics·P M Bartold, R C Page
Nov 1, 1984·The Anatomical Record·R T ParmleyF R Denys
May 1, 1988·Journal of Cellular Biochemistry·R V Iozzo
Nov 1, 1987·Journal of Periodontal Research·P M Bartold
Jul 1, 1997·Revista Do Instituto De Medicina Tropical De São Paulo·F G Melo, C L Rossi
Jan 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·T N Wight
Jan 1, 1984·Cancer Metastasis Reviews·E A Turley

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