Nov 6, 2009

Chondroitin sulfate expression is required for cardiac atrioventricular canal formation

Developmental Dynamics : an Official Publication of the American Association of Anatomists
David S PealDavid J Milan

Abstract

Defects in cardiac valvulogenesis are a common cause of congenital heart disease, and the study of this process promises to provide mechanistic insights and lead to novel therapeutics. Normal valve development involves multiple signaling pathways, and recently roles have been identified for extracellular matrix components, including glycosaminoglycans. We, therefore, explored the role of the glycosaminoglycan chondroitin sulfate during zebrafish cardiac development. Beginning at 33 hr, there is a distinct zone of chondroitin sulfate expression in the atrioventricular (AV) boundary, in the cardiac jelly between the endocardium and myocardium. This expression is both spatially and temporally restricted, and is undetectable after 48 hr. Chemical as well as genetic inhibition of chondroitin synthesis results in AV canal (AVC) defects, including loss of the atrioventricular constriction, blood regurgitation, and failure of circulation. Lack of chondroitin disrupts a marker of cell migration, results in a loss of myocardial and endothelial markers of valvulogenesis, and misregulates bone morphogenetic protein expression, supporting an early role in AVC development. In summary, we have defined a requirement for chondroitin sulfate exp...Continue Reading

  • References38
  • Citations25

Citations

Mentioned in this Paper

Vertebrates
Biochemical Pathway
Chondroitin Sulfate, Zinc Salt
Chondroitin
Entire Atrioventricular Canal
Cardiac Jelly
Glycosaminoglycans
Cell Motility
Persistent Common Atrioventricular Canal
Extracellular Matrix

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