Chondroitin sulfate-functionalized polyamidoamine as a tumor-targeted carrier for miR-34a delivery

Acta Biomaterialia
Wenqi ChenQuanshun Li

Abstract

Chondroitin sulfate (CS) was modified on a polyamidoamine dendrimer (PAMAM) through Michael addition to construct a tumor-targeted carrier CS-PAMAM for miR-34a delivery. The derivative CS-PAMAM was demonstrated to achieve an efficient cellular uptake of miR-34a in a CD44-dependent endocytosis way and further facilitate the endosomal escape of miR-34a after 4h. Through the miR-34a delivery, obvious inhibition of cell proliferation could be detected which was attributed to the enhancement of cell apoptosis and cell cycle arrest, and meanwhile the cell migration and invasion has been observed to be inhibited. Finally, the intravenous injection of CS-PAMAM/miR-34a formulation into mice bearing human lung adenocarcinoma cell A549 xenografts could efficiently inhibit the tumor growth and induce the tumor apoptosis owing to the enhanced accumulation of miR-34a in tumor tissue. Overall, CS-PAMAM is potential to be used as a tumor-targeted oligonucleotide carrier for achieving tumor gene therapy. The cationic dendrimer PAMAM was modified by chondroitin sulfate (CS) through Michael addition to construct a tumor-targeted carrier CS-PAMAM for miR-34a delivery. The introduction of CS could achieve an efficient cellular uptake and intracellu...Continue Reading

Citations

Oct 19, 2017·International Journal of Molecular Sciences·Ammad Ahmad FarooqiAamir Ahmad
Mar 4, 2020·Expert Opinion on Biological Therapy·Vivian Weiwen XueWilliam Chi Shing Cho
Aug 15, 2020·Frontiers in Pharmacology·Priyanka SharmaMarco Tafani
Feb 28, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhiman BaiWei Huang
Jan 13, 2018·Molecular Medicine Reports·Hai-Feng ZhangYi-Di Han
Feb 6, 2021·International Journal of Molecular Sciences·Raphael MohrChristoph Roderburg
Apr 4, 2021·International Journal of Molecular Sciences·Piotr Tarach, Anna Janaszewska
Apr 30, 2021·Drug and Chemical Toxicology·Barbara ZiembaIda Franiak-Pietryga
May 8, 2021·Cancer Metastasis Reviews·Sijing LiLi Sun

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