Chondroitin sulfates act as extracellular gating modifiers on voltage-dependent ion channels

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Davide VigettiAndrea Moriondo

Abstract

Chondroitin sulfates are glycosaminoglycans bound to core proteins of proteoglycans in the extracellular matrix and perineuronal nets surrounding many types of neurones. Chondroitin 4- and chondroitin 6- sulfate can bind calcium ions with different affinities, depending on their sulfation position. Extracellular calcium plays a key role in determining the transmembrane potential sensed by voltage-operated ion channels (VOCs) by means of the "surface screening effect" theory (Gouy-Chapman-Stern theory). We wanted to test whether chondroitin sulfates at physiological concentration can effectively modulate the gating properties of VOCs. We recorded in patch-clamp experiments the shift of h and voltage-dependent calcium currents activation curves of Xenopus laevis photoreceptors perfused with chondroitin sulfate solutions in physiological extracellular calcium concentration. We found that chondroitin 4- and 6- sulfate, with different capabilities, can shift the activation curve of h and voltage-dependent calcium channels, compatibly with the surface screening effect theory. We conclude that chondroitin sulfates can alter VOCs gating by modulating the calcium concentration in the extracellular microenvironment. This phenomenon may e...Continue Reading

Citations

Oct 11, 2012·ACS Chemical Neuroscience·Gerard Marx, Chaim Gilon
Jul 20, 2011·The Journal of Biological Chemistry·Davide VigettiAlberto Passi
Oct 13, 2012·European Journal of Cell Biology·David Denis Sofeu FeugaingManuela Viola
Jan 13, 2015·Hearing Research·Mandy SonntagMarkus Morawski
Sep 8, 2011·Developmental Neurobiology·Jessica C F KwokJames W Fawcett
Jun 26, 2015·Journal of Neurophysiology·Amber E HudsonAstrid A Prinz
Aug 6, 2015·The Journal of Biological Chemistry·Lena EbbersHans Gerd Nothwang
Aug 20, 2019·Advanced Drug Delivery Reviews·Alberto Passi, Davide Vigetti
Jun 16, 2021·Cellular and Molecular Life Sciences : CMLS·Egor DzyubenkoDirk M Hermann
Sep 12, 2018·Chemical Reviews·Nikos K KaramanosRenato V Iozzo

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