May 24, 2012

Chondroitin sulphate and heparan sulphate sulphation motifs and their proteoglycans are involved in articular cartilage formation during human foetal knee joint development

Histochemistry and Cell Biology
James MelroseAnthony J Hayes

Abstract

Novel sulphation motifs within the glycosaminoglycan chain structure of chondroitin sulphate (CS) containing proteoglycans (PGs) are associated with sites of growth, differentiation and repair in many biological systems and there is compelling evidence that they function as molecular recognition sites that are involved in the binding, sequestration or presentation of soluble signalling molecules (e.g. morphogens, growth factors and cytokines). Here, using monoclonal antibodies 3B3(-), 4C3 and 7D4, we examine the distribution of native CS sulphation motifs within the developing connective tissues of the human foetal knee joint, both during and after joint cavitation. We show that the CS motifs have broad, overlapping distributions within the differentiating connective tissues before the joint has fully cavitated; however, after cavitation, they all localise very specifically to the presumptive articular cartilage tissue. Comparisons with the labelling patterns of heparan sulphate (HS), HS-PGs (perlecan, syndecan-4 and glypican-6) and FGF-2, molecules with known signalling roles in development, indicate that these also become localised to the future articular cartilage tissue after joint cavitation. Furthermore, they display inte...Continue Reading

  • References68
  • Citations17

References

  • References68
  • Citations17

Citations

Mentioned in this Paper

Monoclonal Antibodies
Glypican
Monoclonal antibodies, antineoplastic
Chondroitin Sulfate, Zinc Salt
Syndecan-4
Glycosaminoglycans
Proteoglycan
Structure of Articular Cartilage
Mucopolysaccharidosis III
Immunocytochemistry

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