Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo

Biomolecules & Therapeutics
Jin Sung ParkSun-Chul Hwang

Abstract

We evaluated the chondroprotective effects of wogonin by investigating its effects on the gene expression and production of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as on production of MMP-3 in the rat knee. Rabbit articular chondrocytes were cultured in a monolayer, and RT-PCR was used to measure interleukin-1β (IL-1β)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and type II collagen. In rabbit articular chondrocytes, the effects of wogonin on IL-1β-induced production and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The effect of wogonin on MMP-3 protein production was also examined in vivo. In rabbit articular chondrocytes, wogonin inhibited the expression of MMP-3, MMP-1, MMP-13, and ADAMTS-4, but increased expression of type II collagen. Furthermore, wogonin inhibited the production and proteolytic activity of MMP-3 in vitro, and inhibited production of MMP-3 protein in vivo. These results suggest that wogonin can regulate the gene expression and production of MMP-3, by directly acting on articular chondrocytes.

References

Jul 4, 1990·Journal of the National Cancer Institute·P SkehanM R Boyd
Aug 1, 1989·The Journal of Clinical Investigation·D D DeanJ F Woessner
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·H Birkedal-HansenJ A Engler
Feb 16, 2002·Cellular and Molecular Life Sciences : CMLS·T Aigner, L McKenna
Aug 5, 2004·Rheumatology·C S Bonnet, D A Walsh
Apr 1, 2005·Nature·Heather StantonAmanda J Fosang
Sep 9, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Peter S BurrageConstance E Brinckerhoff
Sep 19, 2006·International Immunopharmacology·Chiu-Mei LinJen-Hwey Chiu
Aug 18, 2009·Nature Medicine·Frank EchtermeyerThomas Pap
Jul 17, 2010·Osteoarthritis and Cartilage·I PantsulaiaE Kobyliansky
Nov 26, 2010·Phytotherapy Research : PTR·Peng-fei HuLi-dong Wu
Dec 12, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Yoshiyuki Kimura, Maho Sumiyoshi
Sep 17, 2014·Toxicology and Applied Pharmacology·Soyoung KwakJong-Sup Bae

❮ Previous
Next ❯

Citations

Mar 11, 2017·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Dong-Geun KangJin Sung Park
Jan 10, 2017·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Ho Jong RaSun-Chul Hwang
Nov 23, 2019·BMC Complementary and Alternative Medicine·Doo Jin ChoiGeum Soog Kim
Jan 5, 2017·BMC Complementary and Alternative Medicine·Pallavi S NirmalAbhay M Harsulkar
Mar 7, 2020·Frontiers in Physiology·Jacob F SmithDavid L Kooyman
Oct 21, 2020·International Orthopaedics·Alexander E WeberNicholas A Trasolini
Nov 10, 2020·Molecular Biology Reports·Do Luong HuynhCuong Thach Nguyen
Feb 13, 2021·Antioxidants·Maria-Luisa Pérez-LozanoHechmi Toumi

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.