Choosing a laser for laser speckle contrast imaging

Scientific Reports
Dmitry D PostnovDavid A Boas

Abstract

The use of laser speckle contrast imaging (LSCI) has expanded rapidly for characterizing the motion of scattering particles. Speckle contrast is related to the dynamics of the scattering particles via a temporal autocorrelation function, but the quality of various elements of the imaging system can adversely affect the quality of the signal recorded by LSCI. While it is known that the laser coherence affects the speckle contrast, it is generally neglected in in vivo LSCI studies and was not thoroughly addressed in a practical matter. In this work, we address the question of how the spectral width of the light source affects the speckle contrast both experimentally and through numerical simulations. We show that commonly used semiconductor laser diodes have a larger than desired spectral width that results in a significantly reduced speckle contrast compared with ideal narrow band lasers. This results in a reduced signal-to-noise ratio for estimating changes in the motion of scattering particles. We suggest using a volume holographic grating stabilized laser diode or other diodes that have a spectrum of emitted light narrower than ≈1 nm to improve the speckle contrast.

References

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Dec 25, 2007·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Donald D Duncan, Sean J Kirkpatrick
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Jun 26, 2015·Journal of Biophotonics·Dmitry D PostnovValery V Tuchin
Dec 30, 2015·Biomedical Optics Express·Dmitry D PostnovOlga Sosnovtseva
Jul 26, 2016·Biomedical Optics Express·Anastasiia Y NeganovaOlga Sosnovtseva
Dec 3, 2016·Scientific Reports·Zeinab HajjarianSeemantini K Nadkarni
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Nov 24, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Şefik Evren ErdenerDavid A Boas

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Citations

Feb 12, 2020·Neurophotonics·Smrithi SunilKıvılcım Kılıç
Nov 12, 2019·Cardiovascular Research·Christian StaehrVladimir V Matchkov
Oct 28, 2019·Biomedical Optics Express·Léo PuyoMichael Atlan
Nov 8, 2020·Science Advances·Dmitry D PostnovDavid A Boas
Dec 29, 2020·Applied Optics·Rabisankar Samanta, Sushil Mujumdar
Jul 6, 2021·Biomedical Optics Express·Chang LiuDmitry D Postnov

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Methods Mentioned

BETA
light scattering

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