Chorismate pyruvate-lyase and 4-hydroxy-3-solanesylbenzoate decarboxylase are required for plastoquinone biosynthesis in the cyanobacterium Synechocystis sp. PCC6803.

The Journal of Biological Chemistry
Christian PfaffRadin Sadre

Abstract

Plastoquinone is a redox active lipid that serves as electron transporter in the bifunctional photosynthetic-respiratory transport chain of cyanobacteria. To examine the role of genes potentially involved in cyanobacterial plastoquinone biosynthesis, we have focused on three Synechocystis sp. PCC 6803 genes likely encoding a chorismate pyruvate-lyase (sll1797) and two 4-hydroxy-3-solanesylbenzoate decarboxylases (slr1099 and sll0936). The functions of the encoded proteins were investigated by complementation experiments with Escherichia coli mutants, by the in vitro enzyme assays with the recombinant proteins, and by the development of Synechocystis sp. single-gene knock-out mutants. Our results demonstrate that sll1797 encodes a chorismate pyruvate-lyase. In the respective knock-out mutant, plastoquinone was hardly detectable, and the mutant required 4-hydroxybenzoate for growth underlining the importance of chorismate pyruvate-lyase to initiate plastoquinone biosynthesis in cyanobacteria. The recombinant Slr1099 protein displayed decarboxylase activity and catalyzed in vitro the decarboxylation of 4-hydroxy-3-prenylbenzoate with different prenyl side chain lengths. In contrast to Slr1099, the recombinant Sll0936 protein did n...Continue Reading

References

Jan 1, 1988·Methods in Enzymology·R Rippka
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Oct 2, 2004·Protein Science : a Publication of the Protein Society·Erumbi S RangarajanMiroslaw Cygler
Jun 28, 2005·Nucleic Acids Research·E QuevillonR Lopez
Dec 14, 2005·Archives of Biochemistry and Biophysics·Natasha SmithD T Gallagher
Oct 13, 2006·Acta Biochimica Et Biophysica Sinica·Jun Liu, Jian-Hua Liu
Mar 7, 2007·The Journal of Biological Chemistry·Linnka Lefebvre-LegendreJean-David Rochaix
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Sep 25, 2007·Archives of Biochemistry and Biophysics·Melissa GulmezianGeorge T Javor
Apr 5, 2008·Canadian Journal of Microbiology·Boguslaw LupaJuergen Wiegel
Nov 3, 2009·Nucleic Acids Research·Mitsuteru NakaoYasukazu Nakamura
Apr 20, 2010·The Journal of Biological Chemistry·Radin SadreTim Hawkes
Nov 27, 2010·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Apr 19, 2012·Biology Direct·Grzegorz M BoratynThomas L Madden

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Citations

Apr 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Spencer DiamondSusan S Golden
Jun 1, 2016·Microbiological Research·Beatrycze Nowicka, Jerzy Kruk
Mar 9, 2018·Applied Microbiology and Biotechnology·Songwei WangXuehong Zhang
Nov 7, 2017·Genome Biology and Evolution·Mauro Degli Esposti
Feb 20, 2018·Bioscience, Biotechnology, and Biochemistry·Makoto Kawamukai
Dec 24, 2018·Applied Microbiology and Biotechnology·Beata Żyszka-HaberechtJacek Lipok
May 31, 2019·Nature Communications·Stephen A MarshallDavid Leys
Apr 4, 2021·International Journal of Molecular Sciences·Beatrycze NowickaRenata Szymańska
Jul 15, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Sophie Saphia AbbyFabien Pierrel

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